School of Ocean, Yantai University, Yantai, PR China.
Int J Syst Evol Microbiol. 2024 Jan;74(1). doi: 10.1099/ijsem.0.006256.
In this study, we report a Gram-stain-negative, rod-shaped, atrichous and aerobic bacterial strain named CSW1921, which was isolated from the deep-sea water of a cold seep in South China Sea. Growth of strain CSW1921 occurred at 10.0-35.0 °C (optimum, 30 °C), pH 5.0-10.0 (optimum, pH 8.0-9.0) and with 0-9.0 % (w/v) NaCl (optimum, 1.0-2.0 %). Phylogenetic tree analysis based on 16S rRNA gene sequence or the genomic sequence indicated that strain CSW1921 belonged to the family and was closely related to MA-7-27 (97.5 % sequence similarity). Genomic analysis indicated that strain CSW1921 contains a circular chromosome of 3 592 879 bp with G+C content of 60.5 mol%. The predominant respiratory quinone of CSW1921 was ubiquinone-10. The polar lipids of CSW1921 contained phosphatidylglycerol, three unidentified aminolipids, two unidentified phospholipids and two unidentified lipids. The major fatty acids of strain CSW1921 contained C, C ω7c 11-methyl and summed feature 8 (C ω7c). The average nucleotide identity, DNA-DNA hybridization and average amino acid identity values between strain CSW1921 and members of its related species were 68.02-69.08 %, 12.7-12.9 % and 46.87-48.08 %, respectively, which were lower than the recommended threshold values for bacterial species or genus delineation. Phylogenetic, physiological, biochemical and morphological analyses suggested that strain CSW1921 represents a novel genus and a novel species of the family , and the name gen. nov., sp. nov. is proposed with the type strain CSW1921 (=MCCC 1K08371=KCTC 92834).
在本研究中，我们报告了一株革兰氏阴性、杆状、无鞘和需氧的细菌菌株，命名为 CSW1921，它是从南海冷泉深海水中分离出来的。菌株 CSW1921 的生长温度为 10.0-35.0°C（最佳温度为 30°C），pH 值为 5.0-10.0（最佳 pH 值为 8.0-9.0），盐度为 0-9.0%（w/v）（最佳盐度为 1.0-2.0%）。基于 16S rRNA 基因序列或基因组序列的系统发育树分析表明，菌株 CSW1921 属于 科，与 MA-7-27（97.5%序列相似度）最为密切相关。基因组分析表明，菌株 CSW1921 含有一个 3592879bp 的圆形染色体，G+C 含量为 60.5mol%。CSW1921 的主要呼吸醌是泛醌-10。CSW1921 的极性脂含有磷脂酰甘油、三种未鉴定的胺脂、两种未鉴定的磷脂和两种未鉴定的脂。菌株 CSW1921 的主要脂肪酸含有 C、Cω7c 11-甲基和总和特征 8（Cω7c）。菌株 CSW1921 与相关种之间的平均核苷酸同一性、DNA-DNA 杂交和平均氨基酸同一性值分别为 68.02-69.08%、12.7-12.9%和 46.87-48.08%，均低于细菌种或属划分的推荐阈值。系统发育、生理、生化和形态分析表明，菌株 CSW1921 代表了 科的一个新属和一个新种，建议采用新属名和新种名 ，其模式菌株为 CSW1921（=MCCC 1K08371=KCTC 92834）。
