National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou 510640, China.
Int J Mol Sci. 2023 Sep 22;24(19):14446. doi: 10.3390/ijms241914446.
Rice blast caused by is one of the most serious rice diseases worldwide. The early rice thermosensitive genic male sterile (TGMS) line HD9802S has the characteristics of stable fertility, reproducibility, a high outcrossing rate, excellent rice quality, and strong combining ability. However, this line exhibits poor blast resistance and is highly susceptible to leaf and neck blasts. In this study, backcross introduction, molecular marker-assisted selection, gene chipping, anther culture, and resistance identification in the field were used to introduce the broad-spectrum blast-resistance gene into HD9802S to improve its rice blast resistance. Six induction media were prepared by varying the content of each component in the culture medium. Murashige and Skoog's medium with 3 mg/L 2,4-dichlorophenoxyacetic acid, 2 mg/L 1-naphthaleneacetic acid, and 1 mg/L kinetin and N6 medium with 800 mg/L casein hydrolysate, 600 mg/L proline, and 500 mg/L glutamine could improve the callus induction rate and have a higher green seedling rate and a lower white seedling rate. Compared to HD9802S, two doubled haploid lines containing with stable fertility showed significantly enhanced resistance to rice blast and no significant difference in spikelet number per panicle, 1000-grain weight, or grain shape. Our findings highlight a rapid and effective method for improving rice blast resistance in TGMS lines.
由稻瘟病菌引起的稻瘟病是全球范围内最严重的水稻病害之一。早期的籼稻温敏核雄性不育（TGMS）系 HD9802S 具有育性稳定、可重复性高、杂交率高、稻米品质优良、配合力强等特点。然而，该系表现出较差的抗稻瘟病能力，对叶片和穗颈稻瘟病高度敏感。本研究采用回交导入、分子标记辅助选择、基因打靶、花药培养和田间抗性鉴定等方法，将广谱抗稻瘟病基因 导入 HD9802S，以提高其稻瘟病抗性。通过改变培养基中各成分的含量，制备了 6 种诱导培养基。在 MS 培养基中添加 3 mg/L 2,4-二氯苯氧乙酸、2 mg/L 1-萘乙酸和 1 mg/L 激动素，在 N6 培养基中添加 800 mg/L 水解酪蛋白、600 mg/L 脯氨酸和 500 mg/L 谷氨酰胺，可以提高愈伤组织诱导率，具有较高的绿苗率和较低的白苗率。与 HD9802S 相比，两个含有 并具有稳定育性的双单倍体系对稻瘟病表现出明显增强的抗性，而每穗小穗数、千粒重和粒形无显著差异。我们的研究结果突出了一种快速有效的方法，可用于提高 TGMS 系的稻瘟病抗性。
