School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Int J Mol Sci. 2023 Sep 23;24(19):14462. doi: 10.3390/ijms241914462.
Both inosine and guanosine are precursors of uric acid that may cause the diseases of hyperuricemia and gout in humans. Here, a promising bacterial strain for efficiently biodegrading both inosine and guanosine was successfully isolated from a healthy human intestine and identified as YD01 with 16S rRNA analysis. An initial amount of 49.6 mg·L of inosine or 49.9 mg·L of guanosine was completely removed by YD01 within 12 h, which showed that YD01 had a strong ability to biodegrade inosine and guanosine. Furthermore, the initial amount of 49.2 mg·L of inosine or 49.5 mg·L of guanosine was totally catalyzed by the intracellular crude enzymes of YD01 within 6 h, and the initial inosine amount of 49.6 mg·L or guanosine of 49.7 mg·L was biodegraded by the extracellular crude enzymes of YD01 within 9 h. Illumina Hiseq sequencing and database gene annotation were used to elucidate the genomic characteristics of YD01. Purine nucleoside phosphorylase, encoded by gene 1785, gene 3933, and gene 4403, was found in the KEEG database, which played a crucial role in the biodegradation of inosine and guanosine. The results of this study provide valuable insights into the mechanisms for biodegrading inosine and guanosine using YD01.
肌苷和鸟苷都是尿酸的前体，可能导致人类高尿酸血症和痛风等疾病。在这里，我们从健康人体肠道中成功分离到一株具有高效降解肌苷和鸟苷能力的细菌菌株，并将其鉴定为 YD01，通过 16S rRNA 分析。YD01 在 12 小时内可完全去除 49.6mg·L 的肌苷或 49.9mg·L 的鸟苷，这表明 YD01 具有很强的降解肌苷和鸟苷的能力。此外，YD01 细胞内粗酶在 6 小时内可完全催化 49.2mg·L 的肌苷或 49.5mg·L 的鸟苷，YD01 细胞外粗酶在 9 小时内可完全降解 49.6mg·L 的肌苷或 49.7mg·L 的鸟苷。Illumina Hiseq 测序和数据库基因注释用于阐明 YD01 的基因组特征。在 KEEG 数据库中发现，编码嘌呤核苷磷酸化酶的基因 1785、基因 3933 和基因 4403 发挥了关键作用，可促进肌苷和鸟苷的生物降解。本研究结果为利用 YD01 降解肌苷和鸟苷的机制提供了有价值的见解。
