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具有降尿酸潜力的YC03益生菌特性的基因组分析及体外测定

Genome Analysis and In Vitro Assay of Probiotic Properties of YC03 with Urate-Lowering Potential.

作者信息

Cao Xiaoyu, Zhang Yu, Xu Qianqian, Yan Hai

机构信息

School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.

出版信息

Microorganisms. 2025 Mar 31;13(4):798. doi: 10.3390/microorganisms13040798.

DOI:10.3390/microorganisms13040798
PMID:40284634
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12029181/
Abstract

Hyperuricemia is a metabolic disorder owing to the underexcretion and/or overproduction of uric acid (UA). Recent studies have shown that probiotics have many potential applications as functional foods to ameliorate hyperuricemia. In this study, we have successfully isolated YC03 with urate-lowering potential from Jiangshui. The in vitro results indicated that YC03 exhibited strong biodegradation capacities toward UA and its precursors (inosine and guanosine). Meanwhile, the cell-free extracts of YC03 were also found to catalyze and remove inosine and guanosine. To further explore the application potential of this strain in developing functional foods, we evaluated its probiotic properties through in vitro assays and whole genome analysis. . YC03 has excellent abilities, with tolerance to acid and bile salt and good adhesion. In addition, hemolytic assays, along with antibiotic resistance and biogenic amine production tests, have also preliminarily confirmed the safety of using YC03 in food. We have also annotated the key enzyme genes, including , , , and , which are involved in the biodegradation of UA and nucleosides. The results of nucleoside biodegradation product detection will be valuable for exploring the metabolic pathway for biodegrading nucleosides with YC03. These findings provide meaningful insights for the development of functional foods to improve hyperuricemia using . YC03.

摘要

高尿酸血症是一种由于尿酸（UA）排泄不足和/或生成过多导致的代谢紊乱疾病。最近的研究表明，益生菌作为功能性食品在改善高尿酸血症方面有许多潜在应用。在本研究中，我们成功地从江水分离出具有降尿酸潜力的YC03。体外实验结果表明，YC03对UA及其前体（肌苷和鸟苷）表现出很强的生物降解能力。同时，还发现YC03的无细胞提取物能够催化并去除肌苷和鸟苷。为了进一步探索该菌株在开发功能性食品中的应用潜力，我们通过体外实验和全基因组分析评估了其益生菌特性。YC03具有优异的能力，耐酸和耐胆盐能力强且黏附性良好。此外，溶血实验以及抗生素抗性和生物胺生成测试也初步证实了在食品中使用YC03的安全性。我们还注释了参与UA和核苷生物降解的关键酶基因，包括[具体基因名称未给出]。核苷生物降解产物检测结果对于探索利用YC03生物降解核苷的代谢途径具有重要价值。这些发现为利用YC03开发改善高尿酸血症的功能性食品提供了有意义的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/d53599b6badd/microorganisms-13-00798-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/c97d6386acf6/microorganisms-13-00798-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/880470a5c583/microorganisms-13-00798-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/4b6352ed048e/microorganisms-13-00798-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/cb465412ffd8/microorganisms-13-00798-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/6041647657fe/microorganisms-13-00798-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/c9a8a98ef96f/microorganisms-13-00798-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/d53599b6badd/microorganisms-13-00798-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/c97d6386acf6/microorganisms-13-00798-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/880470a5c583/microorganisms-13-00798-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/4b6352ed048e/microorganisms-13-00798-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/cb465412ffd8/microorganisms-13-00798-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/6041647657fe/microorganisms-13-00798-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/c9a8a98ef96f/microorganisms-13-00798-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb5f/12029181/d53599b6badd/microorganisms-13-00798-g007.jpg

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本文引用的文献

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Foods. 2024 Apr 1;13(7):1082. doi: 10.3390/foods13071082.
2
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Int J Mol Sci. 2023 Sep 23;24(19):14462. doi: 10.3390/ijms241914462.
3
Biodegradation of Uric Acid by -YC02.-YC02对尿酸的生物降解作用
Microorganisms. 2023 Aug 2;11(8):1989. doi: 10.3390/microorganisms11081989.
4
Lactiplantibacillus plantarum enables blood urate control in mice through degradation of nucleosides in gastrointestinal tract.植物乳杆菌通过降解肠道核苷来控制小鼠血尿酸水平。
Microbiome. 2023 Jul 19;11(1):153. doi: 10.1186/s40168-023-01605-y.
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Dairy Products: A Potential Source of Multidrug-Resistant and Strains.乳制品：多重耐药菌和菌株的潜在来源。
Foods. 2022 Dec 19;11(24):4116. doi: 10.3390/foods11244116.
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Probiotic GR-3 ameliorates human hyperuricemia via degrading and promoting excretion of uric acid.益生菌GR-3通过降解和促进尿酸排泄来改善人类高尿酸血症。
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Probiotics, bioactive compounds and dietary patterns for the effective management of hyperuricemia: a review.益生菌、生物活性化合物和饮食模式在高尿酸血症的有效管理中的应用：综述。
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