Diale Mamonokane Olga, Kayitesi Eugenie, Serepa-Dlamini Mahloro Hope
Department of Biotechnology and Food Technology, University of Johannesburg, Johannesburg, South Africa.
Department of Consumer and Food Science, University of Pretoria, Pretoria, South Africa.
Front Genet. 2021 Nov 11;12:672149. doi: 10.3389/fgene.2021.672149. eCollection 2021.
Sporeforming species are gaining interest in human health recently, due to their ability to withstand the harsh environment of the gastrointestinal tract. The present study explores probiotic features of strain MHSD3 through genomic analysis and probiotic assays. The draft genome of strain MHSD3 contained genes associated with tolerance to gastrointestinal stress and adhesion. Cluster genes responsible for the synthesis of antimicrobial non-ribosomal peptide synthetases, bacteriocins, and linear azole-containing peptides were identified. Additionally, strain MHSD3 was able to survive in an acidic environment, had the tolerance to bile salt, and exhibited the capability to tolerate gastric juices. Moreover, the isolate was found to possess strong cell surface traits such as high auto-aggregation and hydrophobicity indices of 79 and 54%, respectively. Gas chromatography-mass spectrometry analysis showed that the strain produced secondary metabolites such as amino acids, phenolic compounds, and organic acid, known to exert health-promoting properties, including the improvement of gastrointestinal tract health.
由于能够耐受胃肠道的恶劣环境，产芽孢物种最近在人类健康领域受到关注。本研究通过基因组分析和益生菌检测探索了菌株MHSD3的益生菌特性。菌株MHSD3的基因组草图包含与胃肠道应激耐受性和黏附相关的基因。鉴定出了负责合成抗菌非核糖体肽合成酶、细菌素和含线性唑肽的聚类基因。此外，菌株MHSD3能够在酸性环境中存活，耐受胆盐，并表现出耐受胃液的能力。此外，该分离株具有较强的细胞表面特性，如高自聚集性，疏水性指数分别为79%和54%。气相色谱-质谱分析表明，该菌株产生了氨基酸、酚类化合物和有机酸等次生代谢产物，这些物质具有促进健康的特性，包括改善胃肠道健康。
