Liu Guimei, Yu Bin, Li Jianpeng, Zhang Zheng, Tao Haiteng, Zhao Haibo, Lu Yanmin, Yuan Chao, Li Quanhong, Cui Bo
State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
National Engineering Research Center for Fruit and Vegetable Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Foods. 2023 Sep 25;12(19):3559. doi: 10.3390/foods12193559.
Herein, we applied the Illumina MiSeq pyrosequencing platform to amplify the V3-V4 hypervariable regions of the 16 S rRNA gene of the gut microbiota (GM) and a gas chromatograph-mass spectrometer to detect the metabolites after supplementation with pumpkin oligosaccharides (POSs) to determine the metabolic markers and mechanisms in rats with type 2 diabetes (T2D). The POSs alleviated glucolipid metabolism by decreasing the serum low-density lipoprotein (LDL), total cholesterol (TC), and glucose levels. These responses were supported by a shift in the gut microbiota, especially in the butyric-acid-producing communities. Meanwhile, elevated total short-chain fatty acid (SCFA), isovaleric acid, and butyric acid levels were observed after supplementation with POSs. Additionally, this work demonstrated that supplementation with POSs could reduce TNF-α and IL-6 secretion via the FFA2-Akt/PI3K pathway in the pancreas. These results suggested that POSs alleviated T2D by changing the SCFA-producing gut microbiota and SCFA receptor pathways.
在此，我们应用Illumina MiSeq焦磷酸测序平台扩增肠道微生物群（GM）16S rRNA基因的V3-V4高变区，并使用气相色谱-质谱仪检测补充南瓜寡糖（POSs）后的代谢产物，以确定2型糖尿病（T2D）大鼠的代谢标志物和机制。POSs通过降低血清低密度脂蛋白（LDL）、总胆固醇（TC）和葡萄糖水平来缓解糖脂代谢。这些反应得到了肠道微生物群的变化的支持，尤其是在产生丁酸的群落中。同时，补充POSs后观察到总短链脂肪酸（SCFA）、异戊酸和丁酸水平升高。此外，这项工作表明，补充POSs可通过胰腺中的FFA2-Akt/PI3K途径减少TNF-α和IL-6的分泌。这些结果表明，POSs通过改变产生SCFA的肠道微生物群和SCFA受体途径来缓解T2D。
