Saeed Taiba, Khan Tanveer Alam, Ahmad Aqeel, Yusuf Mohammad, Kappachery Sajeesh, Fariduddin Qazi, Mudgal Gaurav, Gururani Mayank Anand
Department of Biosciences, Integral University, Kursi Rd., Lucknow 226026, India.
Plant Biotechnology Section, Department of Botany, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India.
Plants (Basel). 2023 Sep 22;12(19):3351. doi: 10.3390/plants12193351.
This study aimed to assess the effects of low-temperature stress on two tomato cultivars (S-22 and PKM-1) treated with 24-epibrassinolide (EBL) and selenium (Se) by determining the changes in the proteomics profiles, growth biomarkers, biochemical parameters, and physiological functions. The growth parameters, photosynthetic traits, and activity of nitrate reductase in the S-22 and PKM-1 plants were markedly reduced by exposure to low temperatures. However, the combined application of EBL and Se under different modes significantly enhanced the aforementioned parameters under stress and non-stress conditions. Exposure to low temperatures increased the activities of the antioxidant enzymes (catalase, peroxidase, and superoxide dismutase) and the proline content of leaves, which were further enhanced by treatment with Se and EBL in both varieties. This research sheds light on the potential for employing exogenous EBL and Se as crucial biochemical tactics to assist tomato plants in surviving low-temperature stress. Moreover, the differentially expressed proteins that were involved in plant metabolism following the combined application of EBL and Se under low-temperature stress were additionally identified. Functional analysis revealed that the Q54YH4 protein plays an active role against plant stressors. The conserved regions in the protein sequences were analyzed for assessing the reliability of plant responses against the external application of EBL and Se under low temperatures.
本研究旨在通过测定蛋白质组学图谱、生长生物标志物、生化参数和生理功能的变化，评估低温胁迫对用24-表油菜素内酯（EBL）和硒（Se）处理的两个番茄品种（S-22和PKM-1）的影响。暴露于低温下，S-22和PKM-1植株的生长参数、光合特性和硝酸还原酶活性显著降低。然而，在不同模式下联合施用EBL和Se在胁迫和非胁迫条件下均显著提高了上述参数。暴露于低温下会增加抗氧化酶（过氧化氢酶、过氧化物酶和超氧化物歧化酶）的活性以及叶片的脯氨酸含量，两个品种经Se和EBL处理后，这些指标进一步提高。本研究揭示了利用外源EBL和Se作为关键生化策略来帮助番茄植株抵御低温胁迫的潜力。此外，还鉴定了低温胁迫下联合施用EBL和Se后参与植物代谢的差异表达蛋白。功能分析表明，Q54YH4蛋白在抵抗植物应激源方面发挥着积极作用。分析了蛋白质序列中的保守区域，以评估低温下植物对外源EBL和Se施用反应的可靠性。
