Lin Chunjing, Duan Yuetong, Li Rong, Wang Pengnian, Sun Yanyan, Ding Xiaoyang, Zhang Jingyong, Yan Hao, Zhang Wei, Peng Bao, Zhao Limei, Zhang Chunbao
Soybean Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033, China.
Key Laboratory of Hybrid Soybean Breeding of the Ministry of Agriculture and Rural Affairs, Changchun 130033, China.
Plants (Basel). 2023 Oct 1;12(19):3461. doi: 10.3390/plants12193461.
(1) Background: Cytoplasmic male sterility (CMS) is important for exploiting heterosis. Soybean ( L.) has a low outcrossing rate that is detrimental for breeding sterile lines and producing hybrid seeds. Therefore, the molecular mechanism controlling the outcrossing rate should be elucidated to increase the outcrossing rate of soybean CMS lines; (2) Methods: The male-sterile soybean lines JLCMS313A (with a high outcrossing rate; HL) and JLCMS226A (with a low outcrossing rate; LL) were used for a combined analysis of the transcriptome (RNA-seq) and the targeted phenol metabolome; (3) Results: The comparison between HL and LL detected 5946 differentially expressed genes (DEGs) and 81 phenolic metabolites. The analysis of the DEGs and differentially abundant phenolic metabolites identified only one common KEGG pathway related to flavonoid biosynthesis. The qRT-PCR expression for eight DEGs was almost consistent with the transcriptome data. The comparison of the cloned coding sequence (CDS) regions of the , , , and genes between HL and LL revealed seven single nucleotide polymorphisms (SNPs) only in the CDS. Moreover, five significant differentially abundant phenolic metabolites between HL and LL were associated with flavonoid metabolic pathways. Finally, on the basis of the SNPs in the CDS, one derived cleaved amplified polymorphic sequence (dCAPS) marker was developed to distinguish between HL and LL soybean lines; (4) Conclusions: The flavonoid biosynthesis pathway may indirectly affect the outcrossing rate of CMS sterile lines in soybean.
(1) 背景：细胞质雄性不育（CMS）对于杂种优势的利用至关重要。大豆（Glycine max (L.) Merr.）的异交率较低，这不利于不育系的选育和杂交种子的生产。因此，应阐明控制异交率的分子机制，以提高大豆CMS系的异交率；(2) 方法：利用雄性不育大豆系JLCMS313A（异交率高；HL）和JLCMS226A（异交率低；LL）进行转录组（RNA测序）和靶向酚类代谢组的联合分析；(3) 结果：HL和LL之间的比较检测到5946个差异表达基因（DEGs）和81种酚类代谢物。对DEGs和差异丰富的酚类代谢物的分析仅确定了一条与类黄酮生物合成相关的常见KEGG途径。8个DEGs的qRT-PCR表达与转录组数据基本一致。HL和LL之间对GmF3H、GmF3'H、GmFLS和GmANS基因的克隆编码序列（CDS）区域进行比较，仅在GmF3'H CDS中发现7个单核苷酸多态性（SNPs）。此外，HL和LL之间5种显著差异丰富的酚类代谢物与类黄酮代谢途径相关。最后，基于GmF3'H CDS中的SNP，开发了一个衍生的酶切扩增多态性序列（dCAPS）标记，以区分HL和LL大豆系；(4) 结论：类黄酮生物合成途径可能间接影响大豆CMS不育系的异交率。
