State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Nutrients. 2023 Sep 27;15(19):4163. doi: 10.3390/nu15194163.
The occurrence of obesity and related metabolic disorders is rising, necessitating effective long-term weight management strategies. With growing interest in the potential role of gut microbes due to their association with responses to different weight loss diets, understanding the mechanisms underlying the interactions between diet, gut microbiota, and weight loss remains a challenge. This study aimed to investigate the potential impact of a multiphase dietary protocol, incorporating an improved ketogenic diet (MDP-i-KD), on weight loss and the gut microbiota. Using metagenomic sequencing, we comprehensively analyzed the taxonomic and functional composition of the gut microbiota in 13 participants before and after a 12-week MDP-i-KD intervention. The results revealed a significant reduction in BMI (9.2% weight loss) among obese participants following the MDP-i-KD intervention. Machine learning analysis identified seven key microbial species highly correlated with MDP-i-KD, with exhibiting the highest response. Additionally, the co-occurrence network of the gut microbiota in post-weight-loss participants demonstrated a healthier state. Notably, metabolic pathways related to nucleotide biosynthesis, aromatic amino acid synthesis, and starch degradation were enriched in pre-intervention participants and positively correlated with BMI. Furthermore, species associated with obesity, such as and , played pivotal roles in regulating these metabolic activities. In conclusion, the MDP-i-KD intervention may assist in weight management by modulating the composition and metabolic functions of the gut microbiota. , and could be key targets for gut microbiota-based obesity interventions.
肥胖症和相关代谢紊乱的发生率不断上升，因此需要有效的长期体重管理策略。由于肠道微生物与不同减肥饮食的反应有关，它们的潜在作用引起了越来越多的关注，因此了解饮食、肠道微生物群和减肥之间相互作用的机制仍然是一个挑战。本研究旨在探讨多相饮食方案（MDP-i-KD）对减肥和肠道微生物群的潜在影响。使用宏基因组测序，我们全面分析了 13 名参与者在 12 周 MDP-i-KD 干预前后肠道微生物群的分类和功能组成。结果表明，MDP-i-KD 干预后肥胖参与者的 BMI 显著降低（体重减轻 9.2%）。机器学习分析确定了与 MDP-i-KD 高度相关的 7 种关键微生物物种，其中 表现出最高的反应。此外，减肥后参与者的肠道微生物群共现网络显示出更健康的状态。值得注意的是，与核苷酸生物合成、芳香族氨基酸合成和淀粉降解相关的代谢途径在干预前参与者中富集，并与 BMI 呈正相关。此外，与肥胖相关的物种，如 和 ，在调节这些代谢活动中发挥着关键作用。总之，MDP-i-KD 干预可能通过调节肠道微生物群的组成和代谢功能来帮助进行体重管理。 、 和 可能是基于肠道微生物群的肥胖干预的关键靶点。
