与脂肪代谢相关的 tRNA 衍生小 RNA 转录组的动态变化。

Dynamic changes in the transcriptome of tRNA-derived small RNAs related with fat metabolism.

机构信息

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.

Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Chengdu, 611130, China.

出版信息

Sci Data. 2023 Oct 14;10(1):703. doi: 10.1038/s41597-023-02624-y.

DOI:10.1038/s41597-023-02624-y

PMID:37838754

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10576826/

Abstract

The prevalence of obesity and overweight is steadily rising, posing a significant global challenge for humanity. The fundamental cause of obesity and overweight lies in the abnormal accumulation of adipose tissue. While numerous regulatory factors related to fat deposition have been identified in previous studies, a considerable number of regulatory mechanisms remain unknown. tRNA-derived small RNAs (tsRNAs), a novel class of non-coding RNAs, have emerged as significant regulators in various biological processes. In this study, we obtained small RNA sequencing data from subcutaneous white adipose tissue and omental white adipose tissue of lean and obese pigs. In addition, we similarly obtained tsRNAs profiles from scapular brown adipose tissue (BAT), inguinal white adipose tissue (iWAT) and epigonadal white adipose tissue (eWAT) of normal mice. Finally, we successfully identified a large number of expressed tsRNAs in each tissue type and identified tsRNAs conserved in different adipose tissues of pigs and mice. These datasets will be a valuable resource for elucidating the epigenetic mechanisms of fat deposition.

摘要

肥胖和超重的患病率正在稳步上升，这对人类构成了重大的全球性挑战。肥胖和超重的根本原因在于脂肪组织的异常积累。虽然之前的研究已经确定了许多与脂肪沉积相关的调节因子，但仍有相当数量的调节机制尚不清楚。tRNA 衍生的小 RNA（tsRNA）作为一类新型非编码 RNA，在各种生物过程中成为重要的调控因子。在这项研究中，我们从瘦猪和肥胖猪的皮下白色脂肪组织和网膜白色脂肪组织中获得了小 RNA 测序数据。此外，我们还从正常小鼠的肩胛骨棕色脂肪组织（BAT）、腹股沟白色脂肪组织（iWAT）和附睾旁白色脂肪组织（eWAT）中获得了 tsRNA 图谱。最后，我们成功地在每种组织类型中鉴定了大量表达的 tsRNA，并鉴定了在猪和鼠不同脂肪组织中保守的 tsRNA。这些数据集将是阐明脂肪沉积的表观遗传机制的宝贵资源。