Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 410005, PR China.
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 410005, PR China.
Food Chem. 2024 Mar 15;436:137702. doi: 10.1016/j.foodchem.2023.137702. Epub 2023 Oct 12.
The intake of estradiol residue from food will lead to health problems, so the rapid and reliable detection of estradiol residue is essential. Multi-mode assays are inherently self-correcting and self-validating, providing more reliable, interference-resistant, high-fidelity results. Here, we developed a dual-mode method to achieve a rapid, reliable, and sensitive detection of estradiol. The binding of thioflavin T to the cavity sites of estradiol aptamer not only generates a strong fluorescence signal, but also provides light-activated oxidase activity to produce a blue oxidation product. But the specific binding between aptamer and estradiol will compete with the above process. Thus a dual-mode fluorescence and colorimetric detection of estradiol was realized with a detection limit of 0.15 μM or 0.27 μM. Besides, the dual-mode method showed a good selectivity for estradiol detection. The method could detect estradiol in meat food, showing a good application potential to monitor endocrine-disrupting chemical.
从食物中摄入雌二醇残留会导致健康问题，因此快速可靠地检测雌二醇残留至关重要。多模式分析具有内在的自我修正和自我验证功能，可以提供更可靠、抗干扰、高保真的结果。在这里，我们开发了一种双模式方法来实现雌二醇的快速、可靠和灵敏检测。硫堇 T 与雌二醇适体的腔位结合不仅会产生强烈的荧光信号，还会提供光激活的氧化酶活性，产生蓝色氧化产物。但适体与雌二醇之间的特异性结合会与上述过程竞争。因此，实现了雌二醇的双模式荧光和比色检测，检测限为 0.15 μM 或 0.27 μM。此外，该双模式方法对雌二醇检测表现出良好的选择性。该方法可以检测肉类食品中的雌二醇，具有很好的应用潜力来监测内分泌干扰化学物质。
