School of Environment and Materials Engineering, Yantai University, Yantai 264005, China.
CAS Key Laboratory of Coastal Environment Processes and Ecological Remediation, Research Center for Coastal Environment Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
Analyst. 2023 Nov 20;148(23):5896-5904. doi: 10.1039/d3an01367g.
Based on surface biomolecular imprinting technology, a rotary microfluidic electrochemical paper-based chip (MIP-ePADs) was proposed for sensitive and selective detection of human interleukin 6 (IL-6) and procalcitonin (PCT). Compared with the traditional method, the sample can be added directly on the MIP-ePAD by rotating the working electrode, which avoids the loss of the liquid to be tested and greatly simplifies the process of electropolymerization imprinting and template elution. Our experimental results show that linear concentration ranges of IL-6 and PCT in the electrochemical molecularly imprinted microfluidic paper-based chip ranged from 0.01 to 5 ng mL, with their detection limits being 3.5 and 2.1 pg mL, respectively. For the detection of actual serum samples, there was no significant difference between the results of MIP-ePADs and the traditional electrochemiluminescence method used in hospitals, indicating that the paper-based chip can be used for stable and accurate analysis and detection. The chip greatly reduces the cost of clinical trials due to its advantages of easy preparation and low cost. The chip can be used for the analysis of non-antibody inflammation markers and can be widely used in home and hospital treatment detection. This method will not only play an important role in rapid detection, but also provide new ideas for the improvement of rapid detection technology.
基于表面生物分子印迹技术，提出了一种旋转微流控电化学纸基芯片（MIP-ePADs），用于灵敏和选择性检测人白细胞介素 6（IL-6）和降钙素原（PCT）。与传统方法相比，通过旋转工作电极可以直接在 MIP-ePAD 上添加样品，这避免了待测试液体的损失，并大大简化了电聚合印迹和模板洗脱的过程。我们的实验结果表明，IL-6 和 PCT 在电化学分子印迹微流控纸基芯片中的线性浓度范围分别为 0.01 至 5ng mL，检测限分别为 3.5 和 2.1pg mL。对于实际血清样品的检测，MIP-ePADs 与医院中使用的传统电化学发光法的结果之间没有显著差异，表明该纸基芯片可用于稳定、准确的分析和检测。由于其易于制备和成本低廉的优势，该芯片大大降低了临床试验的成本。该芯片可用于非抗体炎症标志物的分析，可广泛应用于家庭和医院治疗检测。这种方法不仅将在快速检测中发挥重要作用，而且为快速检测技术的改进提供了新的思路。
