Department of Biological Sciences and Bioengineering, Inha University, Inharo 100, Incheon 22212, Republic of Korea.
Institute for Specialized Teaching and Research, Inha University, Inharo 100, Incheon 22212, Republic of Korea.
Sci Data. 2023 Oct 17;10(1):707. doi: 10.1038/s41597-023-02622-0.
Biofloc technology is increasingly recognised as a sustainable aquaculture method. In this technique, bioflocs are generated as microbial aggregates that play pivotal roles in assimilating toxic nitrogenous substances, thereby ensuring high water quality. Despite the crucial roles of the floc-associated bacterial (FAB) community in pathogen control and animal health, earlier microbiota studies have primarily relied on the metataxonomic approaches. Here, we employed shotgun sequencing on eight biofloc metagenomes from a commercial aquaculture system. This resulted in the generation of 106.6 Gbp, and the reconstruction of 444 metagenome-assembled genomes (MAGs). Among the recovered MAGs, 230 were high-quality (≥90% completeness, ≤5% contamination), and 214 were medium-quality (≥50% completeness, ≤10% contamination). Phylogenetic analysis unveiled Rhodobacteraceae as dominant members of the FAB community. The reported metagenomes and MAGs are crucial for elucidating the roles of diverse microorganisms and their functional genes in key processes such as nitrification, denitrification, and remineralization. This study will contribute to scientific understanding of phylogenetic diversity and metabolic capabilities of microbial taxa in aquaculture environments.
生物絮团技术越来越被认为是一种可持续的水产养殖方法。在该技术中，生物絮团是微生物聚集体产生的，在同化有毒含氮物质方面发挥着关键作用，从而确保了高水质。尽管絮团相关细菌（FAB）群落在病原体控制和动物健康方面发挥着至关重要的作用，但早期的微生物组研究主要依赖于分类群方法。在这里，我们对商业水产养殖系统中的 8 个生物絮团宏基因组进行了鸟枪法测序。这产生了 106.6 Gbp 的数据，重建了 444 个宏基因组组装基因组（MAG）。在回收的 MAG 中，有 230 个是高质量的（≥90%的完整性，≤5%的污染），有 214 个是中质量的（≥50%的完整性，≤10%的污染）。系统发育分析揭示了 Rhodobacteraceae 是 FAB 群落的主要成员。所报道的宏基因组和 MAG 对于阐明不同微生物及其功能基因在硝化、反硝化和再矿化等关键过程中的作用至关重要。这项研究将有助于科学理解水产养殖环境中微生物类群的系统发育多样性和代谢能力。
