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利用深度学习对复杂样本中的植物寄生线虫进行鉴定

Plant Parasitic Nematode Identification in Complex Samples with Deep Learning.

作者信息

Agarwal Sahil, Curran Zachary C, Yu Guohao, Mishra Shova, Baniya Anil, Bogale Mesfin, Hughes Kody, Salichs Oscar, Zare Alina, Jiang Zhe, DiGennaro Peter

机构信息

Department of Electrical & Computer Engineering, University of Florida, Gainesville, Florida, 32611.

Department of Computer & Information Science and Engineering, University of Florida, Gainesville, Florida 32611.

出版信息

J Nematol. 2023 Oct 16;55(1):20230045. doi: 10.2478/jofnem-2023-0045. eCollection 2023 Feb.

Abstract

Plant parasitic nematodes are significant contributors to yield loss worldwide, causing devastating losses to every crop species, in every climate. Mitigating these losses requires swift and informed management strategies, centered on identification and quantification of field populations. Current plant parasitic nematode identification methods rely heavily on manual analyses of microscope images by a highly trained nematologist. This mode is not only expensive and time consuming, but often excludes the possibility of widely sharing and disseminating results to inform regional trends and potential emergent issues. This work presents a new public dataset containing annotated images of plant parasitic nematodes from heterologous soil extractions. This dataset serves to propagate new automated methodologies or speedier plant parasitic nematode identification using multiple deep learning object detection models and offers a path towards widely shared tools, results, and meta-analyses.

摘要

植物寄生线虫是全球作物减产的重要因素,在各种气候条件下,对每一种作物都会造成毁灭性损失。减轻这些损失需要迅速且明智的管理策略,其核心是对田间种群进行识别和量化。目前植物寄生线虫的鉴定方法严重依赖训练有素的线虫学家对手显微镜图像进行人工分析。这种方式不仅昂贵且耗时,还常常排除了广泛分享和传播结果以了解区域趋势和潜在新出现问题的可能性。这项工作展示了一个新的公共数据集,其中包含来自异源土壤提取物的植物寄生线虫的注释图像。该数据集有助于推广新的自动化方法或使用多个深度学习目标检测模型更快地鉴定植物寄生线虫,并为广泛共享工具、结果和元分析提供了一条途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da5f/10578830/4fc8c99f08f0/j_jofnem-2023-0045_fig_001.jpg

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