Faculty of Mathematics and Computer Science, Jagiellonian University, Kraków, Poland.
Ardigen, Kraków, Poland.
PLoS One. 2020 Jun 30;15(6):e0234806. doi: 10.1371/journal.pone.0234806. eCollection 2020.
Preliminary diagnosis of fungal infections can rely on microscopic examination. However, in many cases, it does not allow unambiguous identification of the species due to their visual similarity. Therefore, it is usually necessary to use additional biochemical tests. That involves additional costs and extends the identification process up to 10 days. Such a delay in the implementation of targeted therapy may be grave in consequence as the mortality rate for immunosuppressed patients is high. In this paper, we apply a machine learning approach based on deep neural networks and bag-of-words to classify microscopic images of various fungi species. Our approach makes the last stage of biochemical identification redundant, shortening the identification process by 2-3 days, and reducing the cost of the diagnosis.
真菌感染的初步诊断可以依赖于显微镜检查。然而,在许多情况下,由于它们的外观相似,这并不能明确地鉴定出物种。因此,通常需要使用额外的生化测试。这会增加额外的成本,并将鉴定过程延长至 10 天。对于免疫抑制患者来说,这种靶向治疗的延迟可能会产生严重的后果,因为他们的死亡率很高。在本文中,我们应用了一种基于深度神经网络和词袋的机器学习方法来对各种真菌物种的显微镜图像进行分类。我们的方法使得生化鉴定的最后阶段变得多余,将鉴定过程缩短了 2-3 天,并降低了诊断成本。