Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Int J Comput Assist Radiol Surg. 2024 Mar;19(3):571-579. doi: 10.1007/s11548-023-03026-6. Epub 2023 Oct 19.
Preemptively estimating tissue damage is crucial for a safe surgical procedure. We previously investigated the possibility of estimating the fracture energies of biological tissues based on their elasticities. However, the reason behind the presence of these correlations is poorly understood. In this study, we investigate the effect of a tissue's histology on the correlation between the fracture energy and elasticity. We hypothesize that two tissues with similar fibrous structure will show a similar correlation between the fracture energy and elasticity.
Porcine duodenum were used for this study. Two tensile tests were performed for each porcine duodenum specimen to determine its elasticity and tearing energy. The correlation between fracture energy and elasticity was then investigated using the results from the mechanical tests. Furthermore, duodenum specimens were fixed in 10% formalin while under tension. Microscopic images were then taken to visualize the fibrous structure within the duodenum tissues under tension.
The results from the tensile test showed that the fracture energy had an isotropic positive and linear correlation with the elasticity to the negative 0.5th power (R = 0.89), which was also previously reported in small intestinal (jejunum) specimens. Furthermore, the tearing patterns of the duodenum were identical to the ones reported in the jejunum. Hematoxylin and eosin staining on tissues fixed under tension showed that the endomysium fibers are involved in providing resistance toward traction.
Through mechanical tests, we showed that porcine duodenum tissues also have a correlation between its fracture energy and elasticity. We also discussed that the histological structure of a tissue is an important factor that dictates how the tearing energy of a tissue will correlate to the elasticity. We understood that since the tearing mechanism between the duodenum and jejunum was similar, the correlations between their fracture energies and elasticities were also similar.
预先估计组织损伤对于安全的手术过程至关重要。我们之前研究了基于组织弹性估计生物组织断裂能的可能性。然而，这些相关性存在的原因尚不清楚。在这项研究中，我们研究了组织组织学对断裂能和弹性之间相关性的影响。我们假设具有相似纤维结构的两种组织将表现出断裂能和弹性之间相似的相关性。
本研究使用猪十二指肠。对每个猪十二指肠标本进行两次拉伸测试，以确定其弹性和撕裂能。然后使用力学测试的结果研究断裂能和弹性之间的相关性。此外，在拉伸下将十二指肠标本固定在 10%福尔马林溶液中。然后拍摄微观图像以可视化拉伸下十二指肠组织内的纤维结构。
拉伸测试的结果表明，断裂能与弹性呈各向同性正线性相关，与弹性呈负 0.5 次方相关（R = 0.89），这在小肠（空肠）标本中也有报道。此外，十二指肠的撕裂模式与空肠报道的相同。在拉伸下固定的组织上进行的苏木精和伊红染色显示，肌内膜纤维参与提供抗牵引力。
通过力学测试，我们表明猪十二指肠组织也具有断裂能和弹性之间的相关性。我们还讨论了组织的组织学结构是决定组织撕裂能与弹性相关性的重要因素。我们了解到，由于十二指肠和空肠之间的撕裂机制相似，它们的断裂能和弹性之间的相关性也相似。
