Bgatova N P, Savchenko S V, Lamanov A N, Ergazina M Zh, Adilova M T, Letyagin A Yu
Research Institute of Clinical and Experimental Lymphology - Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Novosibirsk State Medical University, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
Bull Exp Biol Med. 2023 Sep;175(5):667-672. doi: 10.1007/s10517-023-05923-x. Epub 2023 Oct 20.
The ultrastructural organization endotheliocytes of pulmonary blood capillaries in COVID-19 was studied on autopsy material using electron microscopy. Swelling of the cytoplasm and mitochondria with destruction of the cristae, dilation of the Golgi complex cisternae, a decrease in the volume density of the luminal and basal caveolae and free transport vesicles, an increase of the rough endoplasmic reticulum, as well as the presence of elements of coronavirus replication (reticulovesicular structures, zippered endoplasmic reticulum, electron-dense particles in the Golgi cisternae, and vacuoles with viral particles) were revealed. Further studies of the intracellular mechanisms used by the virus to replicate could help to develop antiviral drugs for the treatment of the new coronavirus infection.
利用电子显微镜对新冠疫情死者尸检材料中肺毛细血管内皮细胞的超微结构组织进行了研究。结果显示，细胞质和线粒体肿胀，嵴破坏，高尔基体池扩张，腔面和基底小窝以及游离转运小泡的体积密度降低，粗面内质网增加，同时存在冠状病毒复制元件（网状囊泡结构、拉链状内质网、高尔基体池中的电子致密颗粒以及含有病毒颗粒的空泡）。对病毒用于复制的细胞内机制进行进一步研究，可能有助于开发用于治疗新型冠状病毒感染的抗病毒药物。
