Shojaee Asiyeh, Jahandideh Alireza, Nasrollahi Omran Ayatollah, Sohrabi Haghdoost Nakisa, Khosravi Mehrzad
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Veterinary Surgery, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Curr Med Mycol. 2023 Mar;9(1):14-20. doi: 10.18502/CMM.2023.150672.
Dermatophytosis is one of the most prevalent zoonotic diseases. Increased resistance of dermatophytosis-causing pathogens against antidermatophytic agents highlights the need for alternative medicine with higher efficiency and lower side effects. In the present study, the antifungal activities of different concentrations of methanol extract against , , and were assessed and their efficacy was evaluated in rat dermatophytosis models.
The broth microdilution and well diffusion methods were used to determine the antidermatophytic activity. The study was carried out using 40 dermatophytosis-infected adults male Wistar rats. The animals were divided into 4 groups (5% and 10% ointment, terbinafine, and Vaseline) and treated with ointment until complete recovery. The percentage of wound closure was calculated for each group.
The results revealed that methanol extract was effective to varying extents against the tested dermatophytes. The highest inhibitory activity of was found against with minimum inhibitory concentration and minimum fungicidal concentration values of 4 and 9 µg mL, respectively. The experiment revealed that 10% ointment significantly accelerated skin lesions reduction and completely cured , , and infections after 19, 25, and 38 days, respectively.
The methanol extract of exhibited significant antifungal activity and , suggesting that it could be used as an alternative to antidermatophytic therapy in a dose-dependent manner.
皮肤癣菌病是最常见的人畜共患病之一。引起皮肤癣菌病的病原体对抗皮肤癣菌剂的耐药性增加，凸显了对高效低毒替代药物的需求。在本研究中，评估了不同浓度甲醇提取物对须发癣菌、红色毛癣菌和石膏样小孢子菌的抗真菌活性，并在大鼠皮肤癣菌病模型中评估了其疗效。
采用肉汤微量稀释法和纸片扩散法测定抗皮肤癣菌活性。该研究使用40只感染皮肤癣菌病的成年雄性Wistar大鼠进行。将动物分为4组（5%和10%的提取物软膏、特比萘芬和凡士林），并用软膏治疗直至完全康复。计算每组伤口愈合的百分比。
结果显示，甲醇提取物对受试皮肤癣菌有不同程度的效果。发现提取物对须发癣菌的抑制活性最高，其最低抑菌浓度和最低杀菌浓度分别为4和9μg/mL。实验表明，10%的提取物软膏显著加速了皮肤损伤的减轻，并分别在19、25和38天后完全治愈了须发癣菌、红色毛癣菌和石膏样小孢子菌感染。
提取物的甲醇提取物对须发癣菌和红色毛癣菌表现出显著的抗真菌活性，表明它可以剂量依赖的方式用作抗皮肤癣菌治疗的替代品。
