Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
Microb Ecol. 2023 Nov;86(4):3111-3127. doi: 10.1007/s00248-023-02298-4. Epub 2023 Oct 25.
Intestinal microbiota plays an important role in promoting digestion, metabolism, and immunity. Intestinal microbiota and fatty acids are important indicators to evaluate the health and nutritional composition of Procambarus clarkii. They have been shown to be strongly influence by environmental and genetic factors. However, it is not clear whether environmental factors have a greater impact on the intestinal microbiota and fatty acid composition of crayfish. The link between the intestinal microbial communities and fatty acid (FA) compositions of red swamp crayfish from different geographical has not yet been studied. Thus, the current paper focuses on the influence of different environments on the fatty acids in muscles of crayfish and the possible existence between gut microbiota and fatty acids. Therefore, in this study, we compared the fatty acid compositions and intestinal microbiota of five crayfish populations from different geographical locations. The results were further analyzed to determine whether there is a relationship between geographical location, fatty acid compositions and intestinal microbiota. The gut microbial communities of the crayfish populations were characterized using 16S rRNA high-throughput gene sequencing. The results showed that there were significant differences in FA compositions of crayfish populations from different geographical locations. A similar trend was observed in the gut microbiome, which also varied significantly according to geographic location. Interestingly, the analysis revealed that there was a relationship between fatty acid compositions and intestinal microbes, revealed by alpha diversity analysis and cluster analysis. However, further studies of the interactions between the P. clarkii gut microbiota and biochemical composition are needed, which will ultimately reveal the complexity of microbial ecosystems with potential applications in aquaculture and species conservation.
肠道微生物在促进消化、新陈代谢和免疫方面发挥着重要作用。肠道微生物和脂肪酸是评估克氏原螯虾健康和营养成分的重要指标，它们受到环境和遗传因素的强烈影响。然而，目前尚不清楚环境因素对小龙虾的肠道微生物群和脂肪酸组成的影响更大。不同地理来源的克氏原螯虾肠道微生物群落与脂肪酸（FA）组成之间的联系尚未得到研究。因此，本研究聚焦于不同环境对小龙虾肌肉中脂肪酸的影响以及肠道微生物群和脂肪酸之间可能存在的联系。因此，在这项研究中，我们比较了来自不同地理区域的 5 个小龙虾种群的脂肪酸组成和肠道微生物群。进一步分析这些结果，以确定地理位置、脂肪酸组成和肠道微生物群之间是否存在关系。使用 16S rRNA 高通量基因测序对小龙虾种群的肠道微生物群落进行了表征。结果表明，来自不同地理区域的小龙虾种群的 FA 组成存在显著差异。肠道微生物组也呈现出类似的趋势，根据地理位置的不同也存在显著差异。有趣的是，通过 alpha 多样性分析和聚类分析发现，脂肪酸组成与肠道微生物之间存在关系。然而，需要进一步研究克氏原螯虾肠道微生物群与生化组成之间的相互作用，这将最终揭示微生物生态系统的复杂性，具有水产养殖和物种保护的潜在应用。
