Su Shengyan, Munganga Brian Pelekelo, Du Fukuan, Yu Juhua, Li Jianlin, Yu Fan, Wang Meiyao, He Xinjin, Li Xinyuan, Bouzoualegh Raouf, Xu Pao, Tang Yongkai
Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
Front Microbiol. 2020 Oct 15;11:565267. doi: 10.3389/fmicb.2020.565267. eCollection 2020.
The gut microbiota plays an important role in a variety of physiological functions such as intestinal digestion, metabolic homeostasis, immune response, and responses to disease treatment. Whether there is a relationship between gut microbial communities and fatty acid (FA) profiles of Chinese mitten crab is unclear. Hence, we analyzed the relationship between FA profiles and the gut bacterial communities of six Chinese mitten crab () populations from different lakes. The crabs were sampled from six different lakes in Jiangsu Province, China. The FA profiles of these crab populations were compared and clustered, and then used to determine the relationship between geographic location and FA composition. We also characterized the gut microbial communities of these crabs using 16S rRNA high-throughput gene sequencing. The FA profiles varied significantly ( < 0.05) between crabs from different geographical locations. A similar trend was also observed in the gut microbial communities, which also varied significantly based on their geographical origin ( < 0.05). Furthermore, alpha diversity, cluster analysis, and matching bacterial community structures with specific locations revealed patterns that significantly linked FA profiles to the gut microbiota. Further analysis of FA profiles and gut microbial community generated patterns that linked the two parameters. Hence, it was observed that the gut microbial community seems to contribute significantly to the FA composition of the Chinese mitten crab. However, further studies need to be conducted to investigate the interactions between gut microbial communities and the biochemical composition of the Chinese mitten crab, which will ultimately unravel the complexity of microbial ecosystems for potential applications in aquaculture and species conservation.
肠道微生物群在多种生理功能中发挥着重要作用，如肠道消化、代谢稳态、免疫反应以及对疾病治疗的反应。中华绒螯蟹肠道微生物群落与脂肪酸（FA）谱之间是否存在关联尚不清楚。因此，我们分析了来自不同湖泊的六个中华绒螯蟹（）种群的脂肪酸谱与肠道细菌群落之间的关系。这些螃蟹采自中国江苏省的六个不同湖泊。对这些螃蟹种群的脂肪酸谱进行了比较和聚类，然后用于确定地理位置与脂肪酸组成之间的关系。我们还使用16S rRNA高通量基因测序对这些螃蟹的肠道微生物群落进行了表征。来自不同地理位置的螃蟹之间的脂肪酸谱差异显著（<0.05）。在肠道微生物群落中也观察到了类似的趋势，其也因地理来源而有显著差异（<0.05）。此外，α多样性、聚类分析以及将细菌群落结构与特定位置进行匹配揭示了脂肪酸谱与肠道微生物群之间显著相关的模式。对脂肪酸谱和肠道微生物群落的进一步分析产生了将这两个参数联系起来的模式。因此，观察到肠道微生物群落似乎对中华绒螯蟹的脂肪酸组成有显著贡献。然而，需要进一步开展研究来调查肠道微生物群落与中华绒螯蟹生化组成之间的相互作用，这最终将揭示微生物生态系统的复杂性，以便在水产养殖和物种保护中得到潜在应用。
