Hovind-Hougen K, Birch-Andersen A, Jensen H J
Acta Pathol Microbiol Scand B. 1976 Apr;84(2):101-8. doi: 10.1111/j.1699-0463.1976.tb01909.x.
Cells of Treponema pertenue Gauthier obtained by elution from skin lesions and lymph nodes of experimentally infected hamsters were studied in the electron microscope by means of negative staining. The cells were also examined in thin secions of skin biopsies and lymph nodes. Fimbriae were observed on the negatively stained cells. Until now fimbriae have not been demonstrated on negatively stained cells of other species of Treponema, but at present only one straon of T. pertenue has been studied in our laboratory. Otherwise, the ultrastructure of the T. pertenue cells was found to be very similar, if not identical to, the substructural details observed in cells of T. pallidum and T. cuniculi. In thin sections of skin biopsies, treponemes were observed in the intercellular spaces between cells of the stratum basale and the stratum spinosum layers of the epidermis.
通过从实验感染仓鼠的皮肤病变和淋巴结洗脱获得的 pertenue 密螺旋体（Treponema pertenue Gauthier）细胞，借助负染色法在电子显微镜下进行了研究。还对皮肤活检组织和淋巴结的薄切片中的细胞进行了检查。在负染色的细胞上观察到了菌毛。到目前为止，在其他密螺旋体物种的负染色细胞上尚未证实有菌毛，但目前在我们实验室仅研究了一株 pertenue 密螺旋体。否则，发现 pertenue 密螺旋体细胞的超微结构与梅毒密螺旋体（T. pallidum）和兔密螺旋体（T. cuniculi）细胞中观察到的亚结构细节非常相似，即便不是完全相同。在皮肤活检组织的薄切片中，在表皮基底层和棘层细胞之间的细胞间隙中观察到了密螺旋体。
