Kalish R J, Becker K
Am J Clin Pathol. 1986 Dec;86(6):751-5. doi: 10.1093/ajcp/86.6.751.
The Coulter S-Plus V three-part differential was evaluated for its precision, correlation with 100-cell manual differentials, ability to process routine specimens without flagging, and ability to detect abnormalities as defined by manual differential. The instrument's precision is markedly greater than that of manual methods, especially in the mononuclear category. Correlation with manual differential lymphocyte, mononuclear, and granulocyte percentages was 0.91, 0.54, and 0.94, respectively. Eighty-four percent of routine samples could be processed by the instrument without further review. With the use of a combination of S-Plus V-generated flags and preset laboratory criteria, only 9% of abnormal manual differentials would have been undetected. Three-fourths of these undetected cases involved low-frequency abnormalities of questionable clinical significance. The three-part differential should be able to decrease the number of manual differentials that are performed, which would result in a cost savings to the laboratory.
对库尔特S-Plus V三分群血细胞分析仪进行了评估，内容包括其精密度、与100个细胞手工分类计数的相关性、处理常规标本而无需标记的能力以及检测手工分类计数所定义的异常的能力。该仪器的精密度明显高于手工方法，尤其是在单核细胞类别中。与手工分类计数的淋巴细胞、单核细胞和粒细胞百分比的相关性分别为0.91、0.54和0.94。84%的常规样本可由该仪器处理而无需进一步复查。使用S-Plus V生成的标记和预设的实验室标准相结合的方法，只有9%的异常手工分类计数会未被检测到。这些未被检测到的病例中有四分之三涉及临床意义存疑的低频异常。三分群血细胞分析仪应该能够减少所进行的手工分类计数的数量，这将为实验室节省成本。
