Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovak Republic.
Physiol Res. 2023 Oct 27;72(S3):S287-S297. doi: 10.33549/physiolres.935185.
The rapid development of tissue engineering (TE) and regenerative medicine brings an acute need for biocompatible and bioactive biological scaffolds to regenerate or restore damaged tissue. Great attention is focused on the decellularization of tissues or even whole organs, and the subsequent colonization of such decellularized extracellular matrices by recipient cells. The foreskin is an integral, normal part of the external genitalia that forms the anatomical covering of the glans penis and the urinary meatus of all human and non-human primates. It is mucocutaneous tissue that marks the boundary between mucosa and skin. In this work, we compared two innovative decellularization techniques for human foreskins obtained from donors. We compared the efficacy and feasibility of these protocols and the biosafety of prepared acellular dermal matrixes that can serve as a suitable scaffold for TE. The present study confirms the feasibility of foreskin decellularization based on enzymatic or detergent methods. Both techniques conserved the ultrastructure and composition of natural ECM while being DNA-free and non-toxic, making it an excellent scaffold for follow-up research and TE applications.
组织工程（TE）和再生医学的快速发展带来了对生物相容性和生物活性生物支架的迫切需求，以再生或修复受损组织。人们非常关注组织甚至整个器官的去细胞化，以及随后受体细胞在这种去细胞化细胞外基质中的定植。包皮是外生殖器的一个完整的正常部分，形成所有人类和非人类灵长类动物的阴茎龟头和尿生殖道的解剖覆盖物。它是粘膜和皮肤之间的粘膜组织。在这项工作中，我们比较了两种从供体获得的人类包皮的创新去细胞化技术。我们比较了这些方案的功效和可行性，以及制备的无细胞真皮基质的生物安全性，这些基质可以作为 TE 的合适支架。本研究证实了基于酶或去污剂方法的包皮去细胞化的可行性。这两种技术都保留了天然 ECM 的超微结构和组成，同时是无 DNA 和无毒的，因此是后续研究和 TE 应用的理想支架。
