Cao Zhiqing, Yang Zhenwei, Ye Qunfang, Xiong Juntan, Zhu Aozhou
Department of Pediatric Surgery, Jiangmen Maternity and Child Health Care Hospital, Jiangmen, Guangdong, China.
Department of Urology, Shanghai Eighth People's Hospital, Shanghai, China.
Front Pediatr. 2025 Aug 4;13:1628435. doi: 10.3389/fped.2025.1628435. eCollection 2025.
Hypospadias is a common congenital defect in males, with surgery remaining the primary treatment option. However, urethral reconstruction procedures often require additional tissue transplantation, which is limited by the availability of suitable tissue sources.
In this study, we prepared acellular dermal matrix (ADM) from foreskin obtained through circumcision and isolated urothelium-derived cells from patients with hypospadias. We then evaluated the growth of these urothelium-derived cells on the ADM.
Our results confirmed successful decellularization of the foreskin dermal tissues and demonstrated that the resulting ADM exhibited minimal cytotoxicity toward primary urothelium-derived cells. CFSE and CCK-8 staining assays revealed robust urothelial cell growth on the ADM. Furthermore, the ADM with growing urothelium-derived cells displayed superior biomechanical properties compared to native ADM, suggesting that foreskin ADM is an excellent scaffold for urothelial cell growth.
These findings indicate that foreskin ADM is a promising alternative material for tissue engineering in the treatment of conditions like hypospadias that require urethral reconstruction.
尿道下裂是男性常见的先天性缺陷，手术仍是主要的治疗选择。然而，尿道重建手术通常需要额外的组织移植，这受到合适组织来源可用性的限制。
在本研究中，我们从包皮环切术中获取的包皮制备了脱细胞真皮基质（ADM），并从尿道下裂患者中分离出尿路上皮来源的细胞。然后我们评估了这些尿路上皮来源的细胞在ADM上的生长情况。
我们的结果证实了包皮真皮组织成功脱细胞，并表明所得的ADM对原代尿路上皮来源的细胞表现出最小的细胞毒性。CFSE和CCK-8染色试验显示尿路上皮细胞在ADM上生长旺盛。此外，与天然ADM相比，生长有尿路上皮来源细胞的ADM表现出更好的生物力学性能，这表明包皮ADM是尿路上皮细胞生长的优良支架。
这些发现表明，包皮ADM是一种有前景的替代材料，可用于组织工程，治疗诸如尿道下裂等需要尿道重建的病症。
