Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, 3688 Nanhai Avenue, Nanshan Section, Shenzhen 518060, PR China.
School of Public Health and Management, Chongqing Medical University, Chongqing 400016, PR China.
Int J Syst Evol Microbiol. 2023 Oct;73(10). doi: 10.1099/ijsem.0.006103.
A Gram-stain-negative, facultative anaerobic, non-flagellated and oval-shaped (0.77-0.98 µm wide and 0.74-1.21 µm long) bacterial strain, designated XY-301, was isolated from a marine invertebrate collected from the South China Sea. Strain XY-301 grew at 15-37 °C (optimum, 30-35 °C) and at pH 7.0-8.5 (optimum, pH 8.0). The strain was slightly halophilic and it only grew in the presence of 0.5-6.5 % (w/v) NaCl (optimum, 2.5-3.5 %). Its predominant fatty acid (>10 %) was C 7. The predominant polar lipids of XY-301 were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, six unidentified aminolipids, three unidentified phospholipids and two unknown polar lipids. The respiratory quinone was Q-10. The genome of XY-301 was 4 979 779 bp in size, with a DNA G+C content of 61.3 mol%. The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between XY-301 and SSK3-2 were 73.3, 14.5 and 53.5 %, respectively. Based on the results of phylogenetic, phenotypic, chemotaxonomic and genomic analyses, strain XY-301 is considered to represent a novel species and a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is XY-301 (=KCTC 72212=MCCC 1K03614).
一株革兰氏阴性、兼性厌氧、无鞭毛、椭圆形（0.77-0.98 μm 宽，0.74-1.21 μm 长）的细菌菌株，被命名为 XY-301，从南海采集的海洋无脊椎动物中分离得到。菌株 XY-301 在 15-37°C（最佳温度 30-35°C）和 pH7.0-8.5（最佳 pH8.0）的条件下生长。该菌株略带嗜盐性，仅在 0.5-6.5%（w/v）NaCl（最佳浓度 2.5-3.5%）存在的情况下才能生长。其主要脂肪酸（>10%）为 C7。XY-301 的主要极性脂类为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、六种未鉴定的氨基脂类、三种未鉴定的磷脂类和两种未知的极性脂类。呼吸醌为 Q-10。XY-301 的基因组大小为 4979779 bp，GC 含量为 61.3mol%。XY-301 与 SSK3-2 之间的平均核苷酸同一性、数字 DNA-DNA 杂交和平均氨基酸同一性值分别为 73.3%、14.5%和 53.5%。基于系统发育、表型、化学分类和基因组分析的结果，菌株 XY-301 被认为代表了一个新的科、属，因此提出了一个新的属名， gen. nov.，种名 sp. nov.。模式菌株为 XY-301（=KCTC 72212=MCCC 1K03614）。
