Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, 3688 Nanhai Avenue, Nanshan Section, Shenzhen 518060, PR China.
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, PR China, Chengdu, Sichuan 610041, No. 17, Section 3, South Renmin Road.
Int J Syst Evol Microbiol. 2020 Feb;70(2):1224-1230. doi: 10.1099/ijsem.0.003905.
A novel bacterial strain, XY-99, was isolated from the epidermis of a marine invertebrate of the genus from seawater of the South China Sea. The cells of the strain were aerobic, Gram-stain-negative, non-motile, and oval-shaped (0.8-1.0 µm wide and 1.0-1.5 µm long) without a flagellum. The strain grew at temperatures of 15-37 °C (optimum, 35-37 °C), at pH 5.5-9.5 (optimum 7.5), and at NaCl concentrations of 0-12.0 % (w/v) (optimum 1.5-3.0 %). The major fatty acids (>10 %) were summed feature 8 (comprising Cω7 and/or Cω6), C and 11-methyl Cω7. The predominant polar lipid was diphosphatidylglycerol. The respiratory quinone was Q-10. The closet phylogenetic neighbours were DY470 and JLT1210, showing 97.5 and 97.3 % of 16 s rRNA gene sequence similarity. The genome size of XY-99 was 3 673 499 bp, with 64.5 % DNA G+C content. The average nucleotide identity and digital DNA-DNA hybridization values between XY-99 and DY470 were 72.8 and 14.0 %, respectively, while they were 79.3 and 22.3 % between XY-99 and JLT1210. Characterization based on phylogenetic, phenotypic, chemotaxonomic and genomic evidence demonstrated that XY-99 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XY-99 (=KCTC 72211=MCCC 1K03607).
从南海海水的海洋无脊椎动物属中分离出一株新型细菌菌株 XY-99。该菌株的细胞为需氧、革兰氏阴性、非运动、椭圆形（0.8-1.0μm 宽，1.0-1.5μm 长），无鞭毛。该菌株在 15-37°C（最佳温度为 35-37°C）、pH5.5-9.5（最佳 pH7.5）和 0-12.0%（w/v）的 NaCl 浓度（最佳浓度为 1.5-3.0%）下生长。主要脂肪酸（>10%）为总和特征 8（包含 Cω7 和/或 Cω6）、C 和 11-甲基 Cω7。主要极性脂为双磷脂酰甘油。呼吸醌为 Q-10。与 DY470 和 JLT1210 最接近的系统发育近邻分别为 97.5%和 97.3%的 16S rRNA 基因序列相似性。XY-99 的基因组大小为 3673499bp，GC 含量为 64.5%。XY-99 与 DY470 之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 72.8%和 14.0%，而 XY-99 与 JLT1210 之间的分别为 79.3%和 22.3%。基于系统发育、表型、化学分类和基因组证据的特征表明，XY-99 代表了属的一个新种，提议将其命名为 sp. nov.。模式菌株为 XY-99（=KCTC 72211=MCCC 1K03607）。
