Key Laboratory for Advanced Materials and Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
Department of Medicinal Chemistry, School of Pharmacy, Fudan University, Shanghai 201203, People's Republic of China.
ACS Nano. 2023 Nov 14;17(21):22060-22070. doi: 10.1021/acsnano.3c08767. Epub 2023 Oct 27.
Understanding biological events associated with HS rather than mediated by HS is of great significance but remains to be solved due to a lack of high-integrity imaging tools. In this study, we report a chemoselective probe for HS over HS through the molecular engineering of luminogens. Based on our search for HS-activatable probes with high selectivity, we fabricate water-soluble and biocompatible nanoprobes. Such a designed nanoprobe shows rare aggregation-induced dual-color fluorescence responses to HS, lighting up bright emissions at 588 and 750 nm, respectively. By use of this activatable dual-color fluorescence, high-integrity identification of intracellular HS was successfully realized. Thus, our approach to HS-activated multicolor fluorescent probes could provide valuable insight into interrogating HS-mediated biological events.
理解与 HS 相关而非由 HS 介导的生物学事件具有重要意义，但由于缺乏高完整性的成像工具，这一问题仍有待解决。在这项研究中，我们通过对发光体的分子工程，报告了一种对 HS 具有化学选择性的探针，而不是对 HS 具有化学选择性的探针。基于我们对具有高选择性的 HS 激活探针的寻找，我们制备了水溶性和生物相容性的纳米探针。这种设计的纳米探针对 HS 表现出罕见的聚集诱导双荧光响应，分别发出明亮的 588nm 和 750nm 发射光。通过使用这种激活的双色荧光，可以成功地实现对细胞内 HS 的高完整性识别。因此，我们对 HS 激活的多色荧光探针的方法可以为研究 HS 介导的生物学事件提供有价值的见解。
