Marques Mário Pedro, Neves Beatriz Guapo, Varela Carla, Zuzarte Mónica, Gonçalves Ana Cristina, Dias Maria Inês, Amaral Joana S, Barros Lillian, Magalhães Mariana, Cabral Célia
Clinic Academic Center of Coimbra (CACC), Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal.
Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 3000-548 Coimbra, Portugal.
Pharmaceutics. 2023 Jan 19;15(2):341. doi: 10.3390/pharmaceutics15020341.
(Mill.) Cav., L. and (L.) L. subsp. are representative species of the Côa Valley's flora, a Portuguese UNESCO World Heritage Site. and are traditionally used to preserve olives and to aromatize bonfires on Saint John's Eve, while is mainly used as a spice for river fish dishes. Despite their traditional uses, these aromatic plants are still undervalued, and literature regarding their bioactivity, especially anticancer, is scarce. In this work, the morphology of secretory structures was assessed by scanning electron microscopy (SEM), and the composition of essential oils (EOs) was characterized by gas chromatography-mass spectrometry (GC-MS). The study proceeded with cytotoxic evaluation of EOs in tumor and non-tumor cells with the cell death mechanism explored in glioblastoma (GB) cells. EO presented the most pronounced cytotoxic/antiproliferative activity against tumor cells, with moderate cytotoxicity against non-tumor cells. Whereas, EO exhibited a slightly lower cytotoxic effect against tumor cells and did not affect the viability of non-tumor cells. Meanwhile, EO did not induce a strong cytotoxic effect against GB cells. and EOs lead to cell death by inducing apoptosis in a dose-dependent manner. The present study suggests that and EOs have a strong cytotoxic and antiproliferative potential to be further studied as efficient antitumor agents.
（米尔.）卡瓦、L.和（L.）L.亚种是科阿山谷植物群的代表性物种，科阿山谷是葡萄牙的联合国教科文组织世界遗产地。它们传统上用于保存橄榄以及在圣约翰节前夜为篝火增添香气，而另一种主要用作河鱼菜肴的香料。尽管有这些传统用途，但这些芳香植物仍然未得到充分重视，关于它们生物活性，尤其是抗癌活性的文献很少。在这项工作中，通过扫描电子显微镜（SEM）评估了分泌结构的形态，并通过气相色谱 - 质谱联用（GC - MS）对精油（EOs）的成分进行了表征。该研究接着对肿瘤细胞和非肿瘤细胞进行了精油的细胞毒性评估，并在胶质母细胞瘤（GB）细胞中探索了细胞死亡机制。某种精油对肿瘤细胞呈现出最显著的细胞毒性/抗增殖活性，对非肿瘤细胞具有中等细胞毒性。而另一种精油对肿瘤细胞的细胞毒性作用略低，并且不影响非肿瘤细胞的活力。同时，还有一种精油对GB细胞没有诱导出强烈的细胞毒性作用。另外两种精油通过以剂量依赖的方式诱导细胞凋亡导致细胞死亡。本研究表明，这两种精油具有很强的细胞毒性和抗增殖潜力，有待作为有效的抗肿瘤药物进一步研究。
