Veljovic Tanja, Djuric Milanko, Mirnic Jelena, Gusic Ivana, Maletin Aleksandra, Ivic Stojan, Stojilkovic Marija, Brkic Snezana
Department of Dental Medicine, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia.
Dentistry Clinic of Vojvodina, Department of Dental Medicine, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia.
J Clin Med. 2023 Oct 23;12(20):6688. doi: 10.3390/jcm12206688.
Antioxidant capacity is frequently measured by evaluating superoxide dismutase (SOD) concentration in body fluids. The aim of this study was to compare SOD concentrations in the saliva and plasma of patients with periodontitis to those measured in a group of patients with healthy periodontium, as well as to evaluate the influence of nonsurgical periodontal therapy on salivary and plasma SOD in periodontitis patients. For this purpose, 40 systemically healthy patients aged 30-70 years who had at least 20 teeth were recruited, 20 of whom had periodontitis, and 20 served as healthy periodontitis-free controls. In all participants, periodontal status was assessed via the plaque index (PI), gingival index (GI), papilla bleeding index (PIB), probing depth (PD), and clinical attachment level (CAL), and the SOD concentration in both saliva and plasma was determined by conducting a commercial immunoenzymatic ELISA test. In periodontitis patients, periodontal indices and saliva and blood samples were taken at the beginning of the study, as well as 3 months after periodontal therapy, while in the control group, these data were gathered at the beginning of the study only. SOD values in the saliva of patients with periodontitis (0.244 U/µL) were statistically significantly higher compared with patients with healthy periodontium (0.017 U/µL). Moreover, periodontal therapy led to a statistically significant decrease in this marker in the saliva of patients with periodontitis ( = 0.023), which was comparable with that measured in the control group. On the other hand, no statistically significant differences were noted in plasma SOD values either between the two groups or at follow-up compared with baseline in the group with periodontitis. These findings suggest that the elevated salivary SOD in patients with periodontal disease may represent a mechanism of tissue protection against oxidative stress that occurs in response to periodontal disease.
抗氧化能力通常通过评估体液中超氧化物歧化酶（SOD）浓度来衡量。本研究的目的是比较牙周炎患者唾液和血浆中的SOD浓度与健康牙周组织患者组的测量值，并评估非手术牙周治疗对牙周炎患者唾液和血浆SOD的影响。为此，招募了40名年龄在30 - 70岁、至少有20颗牙齿的全身健康患者，其中20人患有牙周炎，20人作为无牙周炎的健康对照。对所有参与者，通过菌斑指数（PI）、牙龈指数（GI）、龈乳头出血指数（PIB）、探诊深度（PD）和临床附着水平（CAL）评估牙周状况，并通过商业免疫酶联ELISA试验测定唾液和血浆中的SOD浓度。在牙周炎患者中，在研究开始时以及牙周治疗3个月后采集牙周指数、唾液和血液样本，而在对照组中，这些数据仅在研究开始时收集。牙周炎患者唾液中的SOD值（0.244 U/µL）与健康牙周组织患者（0.017 U/µL）相比在统计学上显著更高。此外，牙周治疗导致牙周炎患者唾液中该标志物在统计学上显著降低（ = 0.023），这与对照组测量值相当。另一方面，两组之间以及牙周炎组随访时与基线相比血浆SOD值均未观察到统计学上的显著差异。这些发现表明，牙周病患者唾液中升高的SOD可能代表一种针对牙周病引发的氧化应激的组织保护机制。
