Lau Alice C C, Qiu Yongjin, Moustafa Mohamed Abdallah Mohamed, Nakao Ryo, Shimozuru Michito, Onuma Manabu, Mohd-Azlan Jayasilan, Tsubota Toshio
Laboratory of Wildlife Biology and Medicine, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Hokudai Center for Zoonosis Control in Zambia, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.
Pathogens. 2020 Oct 15;9(10):846. doi: 10.3390/pathogens9100846.
Members of the sensu lato (Bbsl) complex are etiological agents of Lyme disease (LD), and is one of the relapsing fever (RFB). Despite the serological evidence of LD in Malaysia, there has been no report from Sarawak, Malaysian Borneo. Thus, this study aimed to detect and characterize in rodents and ticks from primary forests and an oil palm (OP) plantation in Sarawak. (a member of the Bbsl complex) was detected in 43.8% (14/32) of ; most of the positive ticks were from the OP plantation (13/14). Out of 56 rodents, was detected in four spp. from the OP plantation and was detected in one rodent, , from the primary forest. Further, the positive samples of were randomly selected for multilocus sequence analysis (MLSA). The MLSA results of successfully amplified tick samples revealed a clustering with the sequences isolated from Japan and China. This study is the first evidence of , a known human pathogen in Malaysia, and , which is circulating in ticks and rodents in Sarawak, Malaysian Borneo, and presenting a new geographical record of the spp.
广义（Bbsl）复合体的成员是莱姆病（LD）的病原体，也是回归热（RFB）之一。尽管马来西亚有莱姆病的血清学证据，但马来西亚婆罗洲的砂拉越却没有相关报告。因此，本研究旨在检测和鉴定砂拉越原始森林和油棕种植园中啮齿动物和蜱虫体内的（Bbsl复合体的一个成员）。在32只蜱虫中有43.8%（14/32）检测到了；大多数阳性蜱虫来自油棕种植园（13/14）。在56只啮齿动物中，在油棕种植园的4种啮齿动物中检测到了，在原始森林的1只啮齿动物中检测到了。此外，随机选择阳性样本进行多位点序列分析（MLSA）。成功扩增的蜱虫样本的MLSA结果显示与从日本和中国分离的序列聚类。本研究首次证明了是马来西亚已知的人类病原体，以及在马来西亚婆罗洲砂拉越的蜱虫和啮齿动物中传播，并呈现了该物种的新地理记录。
