Wei Zihao, Dong Yue, Li Xiaolong, Wang Meng, Zhang Keming
State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China.
Foods. 2023 Oct 13;12(20):3767. doi: 10.3390/foods12203767.
This work aimed to clarify the effects of gum arabic (GA) on the morphology and properties of ovotransferrin fibrils (OVTFs). By constructing OVTF-GA complexes and exploring the dispersion stability, turbidity and the ζ-potential of the complexes, the optimum mass ratio of OVTFs to GA and pH for complex formation were confirmed as being 1:1 and pH 4.6, respectively. The interaction between OVTFs and GA was determined to be predominantly driven by electrostatic attraction. The OVTF-GA complexes exhibited a knot-like structure when observed using atomic force microscopy. Then, OVTFs and OVTF-GA complexes were compared in terms of contact angle, surface hydrophobicity and dynamic interfacial tension. The combination of OVTFs and GA decreased the contact angle of OVTFs from 80.85° to 70.36°. In comparison with OVTFs, OVTF-GA complexes reduced the oil-water interfacial tension to a lower level (8.14 mN/m). Furthermore, the capacities of OVTF-GA complexes in stabilizing emulsions were explored. OVTF-GA complex-stabilized oleogel-based Pickering emulsion (OGPE) was constructed, and OVTF-stabilized oleogel-based Pickering emulsion (OPE) was used as the control. OGPE had a higher emulsified phase volume fraction (EPVF) and stability index (SI). The EPVF of OGPE was 100.0% and 99.4% before and after one-month storage, respectively, compared with 98.3% and 95.7% of OPE. This work can provide some useful references for the design of biopolymers with novel structures composed of protein fibrils and polysaccharides, which may also help to construct and apply protein fibril-polysaccharide complexes under specific needs.
本研究旨在阐明阿拉伯胶（GA）对卵转铁蛋白原纤维（OVTFs）形态和性质的影响。通过构建OVTF-GA复合物并探究其分散稳定性、浊度和ζ电位，确定了形成复合物时OVTFs与GA的最佳质量比为1:1，最佳pH值为4.6。研究发现，OVTFs与GA之间的相互作用主要由静电吸引驱动。使用原子力显微镜观察时，OVTF-GA复合物呈现出结状结构。然后，比较了OVTFs和OVTF-GA复合物在接触角、表面疏水性和动态界面张力方面的差异。OVTFs与GA的结合使OVTFs的接触角从80.85°降至70.36°。与OVTFs相比，OVTF-GA复合物将油水界面张力降低到了更低水平（8.14 mN/m）。此外，还探究了OVTF-GA复合物稳定乳液的能力。构建了基于OVTF-GA复合物稳定的油凝胶型皮克林乳液（OGPE），并以基于OVTF稳定的油凝胶型皮克林乳液（OPE）作为对照。OGPE具有更高的乳化相体积分数（EPVF）和稳定性指数（SI）。OGPE在储存一个月前后的EPVF分别为100.0%和99.4%，而OPE的相应值分别为98.3%和95.7%。本研究可为设计由蛋白质原纤维和多糖组成的新型结构生物聚合物提供一些有用的参考，这也可能有助于根据特定需求构建和应用蛋白质原纤维-多糖复合物。
