Zhang Guoqing, Yang Ning, Liu Zhongyuan, Chen Xinyu, Li Mengjiao, Fu Tongyu, Zhang Donghong, Zhao Cuiqing
College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin 132101, China.
Microorganisms. 2023 Sep 22;11(10):2373. doi: 10.3390/microorganisms11102373.
Probiotics gained significant attention for their potential to improve gut health and enhance productivity in animals, including poultry. This comprehensive study focused on the genetic analysis of 18 (LP18) to understand its survival and colonization characteristics in the gastrointestinal tract. LP18 was supplemented in the late-stage diet of laying hens to investigate its impact on growth performance, egg quality, and lipid metabolism. The complete genome sequence of LP18 was determined, consisting of 3,275,044 base pairs with a GC content of 44.42% and two circular plasmids. Genomic analysis revealed genes associated with adaptability, adhesion, and gastrointestinal safety. LP18 supplementation significantly improved the daily laying rate ( < 0.05) during the late-production phase and showed noteworthy advancements in egg quality, including egg shape index ( < 0.05), egg albumen height ( < 0.01), Haugh unit ( < 0.01), and eggshell strength ( < 0.05), with notable improvements in eggshell ultrastructure. Additionally, LP18 supplementation resulted in a significant reduction in serum lipid content, including LDL ( < 0.01), FFA ( < 0.05), and Gly ( < 0.05). These findings provide valuable insights into the genomic characteristics of LP18 and the genes that support its survival and colonization in the gastrointestinal tract. Importantly, this study highlights the potential of LP18 as a probiotic candidate to enhance productivity, optimize egg quality, and modulate lipid metabolism in poultry production.
益生菌因其改善肠道健康和提高包括家禽在内的动物生产性能的潜力而备受关注。这项全面的研究聚焦于对18株（LP18）进行基因分析，以了解其在胃肠道中的存活和定殖特性。在蛋鸡后期日粮中添加LP18，以研究其对生长性能、蛋品质和脂质代谢的影响。确定了LP18的完整基因组序列，由3,275,044个碱基对组成，GC含量为44.42%，还有两个环状质粒。基因组分析揭示了与适应性、黏附性和胃肠道安全性相关的基因。添加LP18显著提高了产蛋后期的日产蛋率（<0.05），并在蛋品质方面取得了显著进展，包括蛋形指数（<0.05）、蛋清高度（<0.01）、哈氏单位（<0.01）和蛋壳强度（<0.05），蛋壳超微结构也有显著改善。此外，添加LP18导致血清脂质含量显著降低，包括低密度脂蛋白（<0.01）、游离脂肪酸（<0.05）和甘油（<0.05）。这些发现为LP18的基因组特征以及支持其在胃肠道中存活和定殖的基因提供了有价值的见解。重要的是，这项研究突出了LP18作为益生菌候选物在提高家禽生产性能、优化蛋品质和调节脂质代谢方面的潜力。
