College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.
Int J Mol Sci. 2023 Oct 14;24(20):15177. doi: 10.3390/ijms242015177.
When the skin is overexposed to ultraviolet rays, free radicals will accumulate in the skin, causing lipid damage and even inducing photoaging of the skin. Combination therapy with antioxidant drugs is a good choice for topical treatment of skin photoaging due to its special physiological structure. In this paper, shikonin was encapsulated in β-cyclodextrin (SH-β-CD) by the precipitation crystallization method, which delayed the release of the drug and increased drug solubility. The average diameter of SH-β-CD was 203.0 ± 21.27 nm with a zeta potential of -14.4 ± 0.5 mV. The encapsulation efficiency (EE%) was 65.9 ± 7.13%. The results of the in vitro permeation across the dialysis membrane and ex vivo transdermal release rates were 52.98 ± 1.21% and 88.25 ± 3.26%, respectively. In vitro antioxidant and antilipid peroxidation model assay revealed the antioxidant potential of SH and SH-β-CD. In the mice model of skin photoaging, SH and SH-β-CD had a recovery effect on the skin damage of mice, which could significantly increase the superoxide dismutase (SOD) activity in the skin. Briefly, SH-β-CD had an obvious therapeutic effect on the skin photoaging of mice caused by UV, and it is promising in skin disease treatment and skin care.
当皮肤过度暴露在紫外线下时，自由基会在皮肤中积累，导致脂质损伤，甚至诱发皮肤光老化。由于其特殊的生理结构，抗氧化药物的联合治疗是皮肤光老化局部治疗的一个不错选择。本文采用沉淀结晶法将紫草素包裹在β-环糊精（SH-β-CD）中，从而延缓药物释放，增加药物溶解度。SH-β-CD 的平均粒径为 203.0±21.27nm，zeta 电位为-14.4±0.5mV。包封率（EE%）为 65.9±7.13%。体外透析膜渗透和离体透皮释放速率的结果分别为 52.98±1.21%和 88.25±3.26%。体外抗氧化和抗脂质过氧化模型检测表明 SH 和 SH-β-CD 具有抗氧化潜力。在皮肤光老化的小鼠模型中，SH 和 SH-β-CD 对小鼠皮肤损伤具有恢复作用，可显著增加皮肤中超氧化物歧化酶（SOD）的活性。简而言之，SH-β-CD 对 UV 引起的小鼠皮肤光老化具有明显的治疗作用，在皮肤病治疗和皮肤护理方面具有广阔的应用前景。
