Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Int J Mol Sci. 2023 Oct 18;24(20):15310. doi: 10.3390/ijms242015310.
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (CRISPR-Cas) system has undergone substantial and transformative progress. Simultaneously, a spectrum of derivative technologies has emerged, spanning both conventional and non-conventional yeast strains. Non-conventional yeasts, distinguished by their robust metabolic pathways, formidable resilience against diverse stressors, and distinctive regulatory mechanisms, have emerged as a highly promising alternative for diverse industrial applications. This comprehensive review serves to encapsulate the prevailing gene editing methodologies and their associated applications within the traditional industrial microorganism, . Additionally, it delineates the current panorama of non-conventional yeast strains, accentuating their latent potential in the realm of industrial and biotechnological utilization. Within this discourse, we also contemplate the potential value these tools offer alongside the attendant challenges they pose.
成簇规律间隔短回文重复序列（CRISPR）和 CRISPR 相关蛋白（CRISPR-Cas）系统取得了重大的变革性进展。与此同时，一系列衍生技术也相继出现，涵盖了传统和非传统的酵母菌株。非传统酵母以其强大的代谢途径、对各种胁迫的强大抵抗力以及独特的调控机制为特点，成为各种工业应用的极具前景的替代选择。本综述旨在概述传统工业微生物体内流行的基因编辑方法及其相关应用。此外，还描绘了非传统酵母菌株的当前概况，强调了它们在工业和生物技术利用领域的潜在潜力。在本文中，我们还探讨了这些工具的潜在价值及其带来的挑战。
