Master Program in Epidemiology and Health Surveillance (PPGEVS), Evandro Chagas Institute (IEC), Ananindeua 67030-000, PA, Brazil.
Master and PhD Program in Parasitic Biology in the Amazon (PPGBPA), University of State of Pará (UEPA), Belém 66087-670, PA, Brazil.
Genes (Basel). 2023 Oct 5;14(10):1907. doi: 10.3390/genes14101907.
COVID-19 is an infectious disease caused by coronavirus 2 of the severe acute syndrome (SARS-CoV-2). Single nucleotide polymorphisms (SNPs) in genes, such as , responsible for an effective human immune response, can change the course of infection. The objective of this article was to verify associations between epidemiological factors and SNP rs3804100 (Thymine [T] > Cytosine [C]) in professionals from Health Institutions (HI) who worked during the first pandemic wave and COVID-19. A case-control study was conducted with Belém-PA HI workers (Northern Brazil), divided into symptomatology groups (Asymptomatic-AS; = 91; and Symptomatic-SI; = 123); and severity groups classified by Chest Computerized Tomography data (symptomatic with pulmonary involvement-SCP; = 35; symptomatic without pulmonary involvement-SSP; = 8). Genotyping was performed by Sanger sequencing, and Statistical Analysis was conducted through the SPSS program. Bioinformatics servers predicted the biological functions of the SNP. There were associations between the presence of comorbidities and poor prognosis of COVID-19 (especially between symptomatology and severity of COVID-19 and overweight and obesity) and between the sickness in family members and kinship (related to blood relatives). The homozygous recessive (C/C) genotype was not found, and the frequency of the mutant allele (C) was less than 10% in the cohort. No significant associations were found for this SNP in this cohort. The presence of SNP was indicated to be benign and causes a decrease in the stability of the TLR2 protein. These data can help the scientific community and medicine find new forms of COVID-19 containment.
COVID-19 是由严重急性综合征（SARS-CoV-2）冠状病毒 2 引起的传染病。负责有效人体免疫反应的基因中的单核苷酸多态性（SNP），如 ，可以改变感染的进程。本文的目的是验证在第一个大流行波期间工作的卫生机构（HI）专业人员中，与流行病学因素和 SNP rs3804100（胸腺嘧啶[T] > 胞嘧啶 [C]）之间的关联，该 SNP 位于负责有效人体免疫反应的基因中。这项研究采用了巴西北部贝伦-帕拉州 HI 工作者（Belém-PA HI workers）进行了病例对照研究，分为有症状组（无症状 AS； = 91；和有症状 SI； = 123）和根据胸部计算机断层扫描数据分类的严重程度组（有肺部受累的有症状 SCP； = 35；无肺部受累的有症状 SSP； = 8）。通过 Sanger 测序进行基因分型，通过 SPSS 程序进行统计分析。生物信息学服务器预测了 SNP 的生物学功能。存在合并症与 COVID-19 的不良预后（尤其是有症状与 COVID-19 的严重程度和超重与肥胖）之间存在关联，与家庭成员患病和亲属关系（与血缘亲属有关）之间存在关联。未发现纯合隐性（C/C）基因型，且在该队列中突变等位基因（C）的频率低于 10%。在该队列中未发现该 SNP 的显著相关性。该 SNP 的存在被认为是良性的，会降低 TLR2 蛋白的稳定性。这些数据可以帮助科学界和医学界找到新的 COVID-19 控制形式。
