大肠杆菌中氨酰基转移核糖核酸合成酶生物合成及转移核糖核酸的调控。V. 缬氨酰转移核糖核酸合成酶水平升高的突变体。
Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. V. Mutants with increased levels of valyl-transfer ribonucleic acid synthetase.
作者信息
Baer M, Low K B, Söll D
出版信息
J Bacteriol. 1979 Jul;139(1):165-75. doi: 10.1128/jb.139.1.165-175.1979.
Abstract
Spontaneous revertants of a temperature-sensitive Escherichia coli strain harboring a thermolabile valyl-transfer ribonucleic acid (tRNA) synthetase were selected for growth at 40 degrees C. Of these, a large number still contain the thermolabile valyl-tRNA synthetase. Three of these revertants contained an increased level of the thermolabile enzyme. The genetic locus, valX, responsible for the enzyme overproduction, is adjacent to the structural gene, valS, of valyl-tRNA synthetase. Determination (by radioimmunoassay) of the turnover rates of valyl-tRNA synthetase showed that the increased level of valyl-tRNA synthetase is due to new enzyme synthesis rather than decreased rates of protein degradation.
摘要
选择了一株携带热不稳定缬氨酰转移核糖核酸（tRNA）合成酶的温度敏感型大肠杆菌菌株的自发回复突变体，使其在40℃下生长。其中，大量回复突变体仍含有热不稳定缬氨酰-tRNA合成酶。这些回复突变体中有三个含有水平升高的热不稳定酶。负责该酶过量产生的基因位点valX与缬氨酰-tRNA合成酶的结构基因valS相邻。通过放射免疫测定法测定缬氨酰-tRNA合成酶的周转率表明，缬氨酰-tRNA合成酶水平的升高是由于新酶的合成，而不是蛋白质降解速率的降低。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Genetic variation in the sex factor of Escherichia coli.大肠杆菌性别因子的遗传变异。
J Bacteriol. 1960 Mar;79(3):321-30. doi: 10.1128/jb.79.3.321-330.1960.
3
Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases.具有改变的亮氨酰和丝氨酰转移核糖核酸合成酶的温度敏感型大肠杆菌突变体的分离及部分特性研究
J Bacteriol. 1971 Nov;108(2):742-50. doi: 10.1128/jb.108.2.742-750.1971.
4
In vivo degradation of mutant lac repressor.突变型乳糖阻遏物的体内降解
Nature. 1970 Dec 19;228(5277):1154-6. doi: 10.1038/2281154a0.
5
6
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.通过与含¹²⁵I的酰化剂结合将蛋白质标记至高比放射性。
Biochem J. 1973 Jul;133(3):529-39. doi: 10.1042/bj1330529.
7
Regulation of synthesis of the branched-chain amino acids and cognate aminoacyl-transfer ribonucleic acid synthetases of Escherichia coli: a common regulatory element.大肠杆菌支链氨基酸及相关氨酰基转移核糖核酸合成酶合成的调控：一种共同调控元件。
J Bacteriol. 1974 Dec;120(3):1380-6. doi: 10.1128/jb.120.3.1380-1386.1974.
8
Metabolic regulation of the arginyl and valyl transfer ribonucleic acid synthetases in bacteria.细菌中精氨酰和缬氨酰转移核糖核酸合成酶的代谢调控
J Biol Chem. 1974 Feb 25;249(4):1044-53.
9
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Eur J Biochem. 1973 Jun;35(2):331-7. doi: 10.1111/j.1432-1033.1973.tb02843.x.
10
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J Bacteriol. 1973 Mar;113(3):1091-5. doi: 10.1128/jb.113.3.1091-1095.1973.
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