Vergnaud Florestan, Mekonnen Benhur, El Abbassi Abdelouahad, Vichery Charlotte, Nedelec Jean-Marie
Université Clermont Auvergne, Clermont Auvergne INP, CNRS, ICCF, F-63000 Clermont-Ferrand, France.
Materials (Basel). 2023 Oct 14;16(20):6690. doi: 10.3390/ma16206690.
Multifunctional substitutes for bone tissue engineering have gained significant interest in recent years in the aim to address the clinical challenge of treating large bone defects resulting from surgical procedures. Sol-gel mesoporous bioactive glass nanoparticles (MBGNs) have emerged as a promising solution due to their high reactivity and versatility. The effect of calcium content on MBGNs textural properties is well known. However, the relationship between their composition, textural properties, and reactivity has not yet been thoroughly discussed in existing studies, leading to divergent conclusions. In this study, pristine and copper-doped binary MGBNs were synthesized by a modified Stöber method, using a cationic surfactant as pore-templating agent. An opposite evolution between calcium content (12-26 wt%) and specific surface area (909-208 m/g) was evidenced, while copper introduction (8.8 wt%) did not strongly affect the textural properties. In vitro bioactivity assessments conducted in simulated body fluid (SBF) revealed that the kinetics of hydroxyapatite (HAp) crystallization are mainly influenced by the specific surface area, while the composition primarily controls the quantity of calcium phosphate produced. The MBGNs exhibited a good bioactivity within 3 h, while Cu-MBGNs showed HAp crystallization after 48 h, along with a controlled copper release (up to 84 ppm at a concentration of 1 mg/mL). This comprehensive understanding of the interplay between composition, textural properties, and bioactivity, offers insights for the design of tailored MBGNs for bone tissue regeneration with additional biological and antibacterial effects.
近年来，用于骨组织工程的多功能替代物引起了广泛关注，旨在应对手术导致的大骨缺损这一临床挑战。溶胶-凝胶介孔生物活性玻璃纳米颗粒（MBGNs）因其高反应活性和多功能性而成为一种有前景的解决方案。钙含量对MBGNs结构性质的影响是众所周知的。然而，现有研究尚未对其组成、结构性质和反应活性之间的关系进行深入讨论，导致结论不一。在本研究中，采用改进的Stöber方法，以阳离子表面活性剂作为孔模板剂，合成了原始的和铜掺杂的二元MGBNs。结果表明，钙含量（12-26 wt%）与比表面积（909-208 m/g）呈相反的变化趋势，而铜的引入（8.8 wt%）对结构性质影响不大。在模拟体液（SBF）中进行的体外生物活性评估表明，羟基磷灰石（HAp）结晶动力学主要受比表面积影响，而组成主要控制磷酸钙的生成量。MBGNs在3小时内表现出良好的生物活性，而Cu-MBGNs在48小时后出现HAp结晶，并伴有可控的铜释放（浓度为1 mg/mL时高达84 ppm）。对组成、结构性质和生物活性之间相互作用的这种全面理解，为设计具有额外生物学和抗菌作用的定制MBGNs用于骨组织再生提供了思路。
