Kesse Xavier, Vichery Charlotte, Jacobs Aurelie, Descamps Stéphane, Nedelec Jean-Marie
Université Clermont Auvergne, CNRS, SIGMA Clermont, ICCF, F-63000 Clermont-Ferrand, France.
ACS Appl Bio Mater. 2020 Feb 17;3(2):1312-1320. doi: 10.1021/acsabm.0c00036. Epub 2020 Jan 31.
Sol-gel-derived bioactive glass nanoparticles (BGNs) are fascinating materials for bone regeneration. In the literature, it can be found that their specific surface area and their calcium content (Ca/Si ratio) are the two key parameters impacting strongly the particles' bioactivity. Nevertheless, in most studies, bioactivity tests are performed on a series of materials where both the composition and the specific surface area are varied. It is thus difficult to unravel the effect of each parameter independently. In this study, spherical and monodispersed BGNs with different Ca/Si ratios and a similar specific surface area have been synthesized by a modified Stöber method in order to specify the impact of the calcium content only. The mineralization studies performed in simulated body fluid showed that the bioactivity increases with the amount of calcium incorporated in the glass matrix. However, this effect is not significant in the composition interval studied (7-15% mol of CaO). Such a result proves that the effective Ca/Si ratio is not the major parameter that affects the bioactivity of sol-gel binary BGs. biocompatibility assessment during 3 and 7 days using human mesenchymal stem cells in contact with the sample showing the fastest mineralization proved its noncytotoxicity. Hence, biomedical applications can be intended for this sample.
溶胶-凝胶法制备的生物活性玻璃纳米颗粒（BGNs）是用于骨再生的迷人材料。在文献中可以发现，它们的比表面积和钙含量（Ca/Si比）是强烈影响颗粒生物活性的两个关键参数。然而，在大多数研究中，生物活性测试是在一系列组成和比表面积都变化的材料上进行的。因此，很难独立地揭示每个参数的影响。在本研究中，通过改进的Stöber方法合成了具有不同Ca/Si比和相似比表面积的球形且单分散的BGNs，以便仅确定钙含量的影响。在模拟体液中进行的矿化研究表明，生物活性随着玻璃基质中钙含量的增加而增加。然而，在所研究的组成区间（7-15%摩尔的CaO）内，这种影响并不显著。这样的结果证明，有效Ca/Si比不是影响溶胶-凝胶二元生物玻璃生物活性的主要参数。使用与矿化最快的样品接触的人间充质干细胞进行3天和7天的生物相容性评估，证明了其无细胞毒性。因此，该样品可用于生物医学应用。
