de Bont J A, Vorage M J, Hartmans S, van den Tweel W J
Appl Environ Microbiol. 1986 Oct;52(4):677-80. doi: 10.1128/aem.52.4.677-680.1986.
A gram-negative, peritrichously flagellated rod, tentatively identified as an Alcaligenes sp., was isolated from a mixture of soil and water samples by using 1,3-dichlorobenzene as the sole carbon and energy source. During growth on 1,3-dichlorobenzene, almost stoichiometric amounts of chloride were released. Simultaneous adaptation studies, as well as enzyme studies, indicated that 1,3-dichlorobenzene was metabolized via 3,5-dichloro-cis-1,2-dihydroxycyclohexa-3,5-diene to 3,5-dichlorocatechol. Subsequently, the latter product was cleaved, yielding 2,4-dichloromuconate. No initial hydrolytic step yielding 3-chlorophenol was detected in this species.
从土壤和水样混合物中分离出一株革兰氏阴性、周生鞭毛杆菌,初步鉴定为产碱菌属。该菌以1,3-二氯苯作为唯一碳源和能源。在以1,3-二氯苯为底物生长过程中,几乎按化学计量比释放出氯离子。同步适应性研究以及酶学研究表明,1,3-二氯苯通过3,5-二氯-顺-1,2-二羟基环己-3,5-二烯代谢为3,5-二氯儿茶酚,随后后者被裂解,生成2,4-二氯粘康酸。在该菌株中未检测到产生3-氯苯酚的初始水解步骤。