Grupo de Biopolímeros y Nanoestructuras, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí 78210, Mexico; Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina, Universidad Autónoma de San Luis Potosí, Av. Sierra Leona 550, Lomas 2a. sección, San Luis Potosí 78210, Mexico.
Grupo de Biopolímeros y Nanoestructuras, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, San Luis Potosí 78210, Mexico.
Int J Biol Macromol. 2024 Jan;254(Pt 1):127691. doi: 10.1016/j.ijbiomac.2023.127691. Epub 2023 Oct 28.
Among the conditions caused by diabetes, the diabetic foot is a significant public health problem due to its delayed healing process. That makes it essential to design, manufacture, and apply auxiliary dressings during healing. In this work, chitosan sponges were developed and evaluated as wound dressings. Metformin, fucoidan, and exopolysaccharide from Porphyridium purpureum algae were loaded into the sponges and studied as healing promoters. The composite sponges were physicochemically, morphologically, and thermally characterized, allowing us to determine the chemical mechanisms involved in the sponge formation. The mechanical analysis demonstrated that sponge composites have shape memory and good mechanical performance under compression stress, showing a compressive strength above 30 kPa. These results correlated with the materials' porosity, influencing the swelling capacity that reached a maximum of 70 %. The morphology of materials was observed by SEM, resulting in folded films with surface porosity. The results of the biocompatibility tests confirmed that the materials are not cytotoxic or hemolytic and have good antibacterial activity. In vivo wound healing evaluation showed that metformin-loaded chitosan sponges regenerated skin tissue after 21 days of treatment, highlighting the rate of healing provided when exopolysaccharide was added to promote tissue regeneration, which can be corroborated by histological analysis. These results make chitosan sponge compounds promising dressings for diabetic foot wound treatment.
在糖尿病引起的各种病症中，糖尿病足是一个严重的公共卫生问题，因为它的愈合过程比较缓慢。这就需要在治疗过程中设计、制造和应用辅助敷料。在这项工作中，壳聚糖海绵被开发并评估为伤口敷料。二甲双胍、褐藻糖胶和紫球藻的胞外多糖被负载到海绵中，并作为愈合促进剂进行研究。复合海绵进行了物理化学、形态和热特性分析，使我们能够确定海绵形成中涉及的化学机制。机械分析表明，海绵复合材料在压缩应力下具有形状记忆和良好的机械性能，压缩强度高于 30kPa。这些结果与材料的孔隙率相关，影响了达到最大 70%的溶胀能力。通过 SEM 观察到材料的形态，得到了具有表面孔隙率的折叠膜。细胞相容性试验的结果证实，这些材料无细胞毒性或溶血作用，并且具有良好的抗菌活性。体内伤口愈合评估表明，载有二甲双胍的壳聚糖海绵在治疗 21 天后再生了皮肤组织，这突出了加入胞外多糖促进组织再生时提供的愈合速度，可以通过组织学分析得到证实。这些结果使壳聚糖海绵化合物成为治疗糖尿病足伤口的有前途的敷料。
