Mansour Fotouh R, Abdallah Inas A, Bedair Alaa, Hamed Mahmoud
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Tanta University, Tanta 31111, Egypt.
Department of Analytical Chemistry, Faculty of Pharmacy, University of Sadat City, Sadat City 32897, Monufia, Egypt.
Crit Rev Anal Chem. 2025;55(1):187-212. doi: 10.1080/10408347.2023.2269421. Epub 2023 Oct 29.
Flavonoids are plant-derived compounds that have several health benefits, including antioxidative, anti-inflammatory, anti-mutagenic, and anti-carcinogenic effects. Quercetin is a flavonoid that is widely present in various fruits, vegetables, and drinks. Accurate determination of quercetin in different samples is of great importance for its potential health benefits. This review, is an overview of sample preparation and determination methods for quercetin in diverse matrices. Previous research on sample preparation and determination methods for quercetin are summarized, highlighting the advantages and disadvantages of each method and providing insights into recent developments in quercetin sample treatment. Various analytical techniques are discussed including spectroscopic, chromatographic, electrophoretic, and electrochemical methods for the determination of quercetin and its derivatives in different samples. UV-Vis (Ultraviolet-visible) spectrophotometry is simple and inexpensive but lacks selectivity. Chromatographic techniques (HPLC, GC) offer selectivity and sensitivity, while electrophoretic and electrochemical methods provide high resolution and low detection limits, respectively. The aim of this review is to comprehensively explore the determination methods for quercetin and quercetin glycosides in diverse matrices, with emphasis on pharmaceutical and biological samples. The review also provides a theoretical basis for method development and application for the analysis of quercetin and quercetin glycosides in real samples.
黄酮类化合物是源自植物的化合物，具有多种健康益处，包括抗氧化、抗炎、抗诱变和抗癌作用。槲皮素是一种广泛存在于各种水果、蔬菜和饮品中的黄酮类化合物。准确测定不同样品中的槲皮素对于其潜在的健康益处至关重要。本综述概述了不同基质中槲皮素的样品制备和测定方法。总结了先前关于槲皮素样品制备和测定方法的研究，突出了每种方法的优缺点，并对槲皮素样品处理的最新进展提供了见解。讨论了各种分析技术，包括用于测定不同样品中槲皮素及其衍生物的光谱法、色谱法、电泳法和电化学方法。紫外可见分光光度法简单且成本低，但缺乏选择性。色谱技术（高效液相色谱法、气相色谱法）具有选择性和灵敏度，而电泳法和电化学方法分别提供高分辨率和低检测限。本综述的目的是全面探索不同基质中槲皮素和槲皮素糖苷的测定方法，重点是药物和生物样品。该综述还为实际样品中槲皮素和槲皮素糖苷分析的方法开发和应用提供了理论基础。
