用于检测登革热抗体的免疫球蛋白G和M特异性酶联免疫吸附测定
Immunoglobulin G- and M-specific enzyme-linked immunosorbent assay for detection of dengue antibodies.
作者信息
Dittmar D, Cleary T J, Castro A
出版信息
J Clin Microbiol. 1979 Apr;9(4):498-502. doi: 10.1128/jcm.9.4.498-502.1979.
Abstract
An enzyme-linked immunosorbent assay for the detection of antibodies to dengue virus is described. This method correlates well with a hemagglutination inhibition technique. The enzyme-linked immunosorbent assay can also be specific for human immunoglobulin M antibodies when a mu-chain-specific antiglobulin-enzyme conjugate and fractionated serum are employed. By using this technique, dengue immunoglobulin M antibodies were demonstrated in an infant suspected of having a recent dengue infection.
摘要
本文描述了一种用于检测登革病毒抗体的酶联免疫吸附测定法。该方法与血凝抑制技术相关性良好。当使用μ链特异性抗球蛋白 - 酶结合物和分级血清时，酶联免疫吸附测定法对人免疫球蛋白M抗体也具有特异性。通过使用该技术，在一名疑似近期感染登革热的婴儿体内检测到了登革免疫球蛋白M抗体。
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