Lopes Dos Santos Rogério, Malo Michel, Campillo Clément
Université Paris-Saclay, Univ Evry, CY Cergy Paris Université, CNRS, LAMBE, 91025 Evry, Courcouronnes, France.
Institut Universitaire de France (IUF), 75005 Paris, France.
ACS Synth Biol. 2023 Nov 17;12(11):3267-3274. doi: 10.1021/acssynbio.3c00268. Epub 2023 Nov 1.
Deciphering the physical mechanisms underlying cell shape changes, while avoiding the cellular interior's complexity, involves the development of controlled basic biomimetic systems that imitate cell functions. In particular, the reconstruction of cytoskeletal dynamics on cell-sized giant unilamellar vesicles (GUVs) has allowed for the reconstituting of some cell-like processes . In fact, such a bottom-up strategy could be the basis for forming protocells able to reorganize or even move autonomously. However, reconstituting the subtle and controlled dynamics of the cytoskeleton-membrane interface remains an experimental challenge. Taking advantage of the lipid-induced segregation of an actin polymerization activator, we present a system that targets actin polymerization in specific domains of phase-separated GUVs. We observe actin networks localized on Lo, Ld, or on both types of domains and the actin-induced deformation or reorganization of these domains. These results suggest that the system we have developed here could pave the way for future experiments further detailing the interplay between actin dynamics and membrane heterogeneities.
在避免细胞内部复杂性的同时，解析细胞形状变化背后的物理机制，需要开发能够模仿细胞功能的可控基础仿生系统。特别是，在细胞大小的巨型单层囊泡（GUVs）上重建细胞骨架动力学，使得一些类似细胞的过程得以重构。事实上，这种自下而上的策略可能是形成能够自主重组甚至移动的原始细胞的基础。然而，重建细胞骨架-膜界面的微妙且可控的动力学仍然是一项实验挑战。利用脂质诱导的肌动蛋白聚合激活剂的分离，我们展示了一个针对相分离GUVs特定区域中肌动蛋白聚合的系统。我们观察到肌动蛋白网络定位于Lo、Ld或这两种类型的区域上，以及这些区域因肌动蛋白诱导的变形或重组。这些结果表明，我们在此开发的系统可能为未来进一步详细研究肌动蛋白动力学与膜异质性之间相互作用的实验铺平道路。
