Xie Yong, Wang Xiaoyan, Wang Zhonghui, Feng Jianyang, Li Dongsheng
Department of Obstetrics and Gynecology, The First People's Hospital of Foshan, Foshan 528000, China.
Department of Obstetrics and Gynecology, Leping Affiliated Hospital of the First People's Hospital of Foshan, Foshan 528100, China.
Heliyon. 2023 Oct 16;9(10):e21075. doi: 10.1016/j.heliyon.2023.e21075. eCollection 2023 Oct.
This study aimed to investigate the effects of mesenchymal stem cell exosomes loaded with FeO magnetic particles (FeO@ MSC-exo) on the survival and neural differentiation of PC12 cells. Exosomes were separated from FeO magnetic nanoparticles-primed umbilical cord mesenchymal stem cells condition medium by ultracentrifugation and characterized by transmission electron microscopy, flow nano analysis, and western blotting. PC12 cells were treated with culture medium containing exosomes. The effects of FeO@ MSC-exo on PC12 cell proliferation, migration, and neural differentiation were analyzed using CCK-8 assay, transwell migration assay, RT-qPCR, and immunofluorescence, respectively. Additionally, miRNA sequencing was performed on FeO@ MSC-exo, followed by bioinformatic analysis of the results. We found that FeO@ MSC-exo can promote PC12 cell proliferation, migration, and neural differentiation. According to the sequencing results, there were a total of 43 differentially expressed miRNAs. The present study indicated that FeO@ MSC-exo might enhance nerve cell function, laying the foundation for targeted therapy of nerve injury.
本研究旨在探讨负载FeO磁性颗粒的间充质干细胞外泌体（FeO@MSC-exo）对PC12细胞存活及神经分化的影响。通过超速离心从负载FeO磁性纳米颗粒的脐带间充质干细胞条件培养基中分离外泌体，并采用透射电子显微镜、流式纳米分析和蛋白质免疫印迹法对其进行表征。用含有外泌体的培养基处理PC12细胞。分别采用CCK-8法、Transwell迁移实验、RT-qPCR和免疫荧光法分析FeO@MSC-exo对PC12细胞增殖、迁移及神经分化的影响。此外，对FeO@MSC-exo进行了miRNA测序，并对结果进行生物信息学分析。我们发现FeO@MSC-exo可促进PC12细胞增殖、迁移及神经分化。根据测序结果，共有43个差异表达的miRNA。本研究表明，FeO@MSC-exo可能增强神经细胞功能，为神经损伤的靶向治疗奠定基础。
