Ratge D, Gehrke A, Melzner I, Wisser H
Clin Exp Pharmacol Physiol. 1986 Jul;13(7):543-53. doi: 10.1111/j.1440-1681.1986.tb00937.x.
To investigate changes of free and sulfoconjugated catecholamines in response to alterations in sympatho-adrenal activity, free and conjugated noradrenaline, adrenaline and dopamine were determined radioenzymatically in plasma of 49 subjects. During brief vigorous bicycle exercise (8 min, maximal heart rate: 177 beats/min) mean free noradrenaline and adrenaline values of 2.0 and 0.51 nmol/l at rest, increased to 6.7 and 2 nmol/l (P less than 0.001) respectively, at the maximal workload of 200 watt, whereas conjugated noradrenaline and adrenaline decreased from 3.4 and 0.8 nmol/l to 2.1 and 0.4 nmol/l (P less than 0.001) respectively. In the tenth min of the recovery period basal free and conjugated noradrenaline and adrenaline levels were measured. The moderate stress of a steam bath (20 min, maximal heart rate: 131 beats/min) doubled free noradrenaline and adrenaline levels. However, conjugated noradrenaline and adrenaline concentrations remained unchanged. The increase in free catecholamine values during an exhausting cross-country march over 20 km was associated with an accumulation of sulfated catecholamines. After a rest of 30 min free noradrenaline and adrenaline reached basal values, whereas conjugated noradrenaline and adrenaline remained elevated by 64 and 70% respectively, compared to pre-exercise concentrations. It was concluded that conjugated noradrenaline and adrenaline may be used as pools for free noradrenaline and adrenaline during brief vigorous exercise. In addition, they may also be indicators of chronic activation of the sympatho-adrenal system.
为研究游离和硫酸结合儿茶酚胺随交感 - 肾上腺活动改变的变化情况，采用放射酶法测定了49名受试者血浆中游离和结合的去甲肾上腺素、肾上腺素及多巴胺。在短暂剧烈自行车运动（8分钟，最大心率：177次/分钟）期间，静息时游离去甲肾上腺素和肾上腺素的平均水平分别为2.0和0.51纳摩尔/升，在200瓦最大负荷时分别升至6.7和2纳摩尔/升（P＜0.001），而结合去甲肾上腺素和肾上腺素则分别从3.4和0.8纳摩尔/升降至2.1和0.4纳摩尔/升（P＜0.001）。在恢复期第10分钟测量基础游离和结合去甲肾上腺素及肾上腺素水平。蒸汽浴（20分钟，最大心率：131次/分钟）的中度应激使游离去甲肾上腺素和肾上腺素水平加倍。然而，结合去甲肾上腺素和肾上腺素浓度保持不变。在超过20公里的越野耐力行军期间，游离儿茶酚胺值的增加与硫酸化儿茶酚胺的蓄积有关。休息30分钟后，游离去甲肾上腺素和肾上腺素恢复至基础值，而结合去甲肾上腺素和肾上腺素与运动前浓度相比分别仍升高64%和70%。得出的结论是，在短暂剧烈运动期间，结合去甲肾上腺素和肾上腺素可能作为游离去甲肾上腺素和肾上腺素的储备库。此外，它们也可能是交感 - 肾上腺系统慢性激活的指标。
