School of Stomatology, Lanzhou University, Lanzhou, 730000, P. R. China.
Key Laboratory of Dental Maxillofacial Reconstruction and Biological Intelligence Manufacturing, Gansu Province, Lanzhou, 730000, P. R. China.
Adv Healthc Mater. 2024 Jan;13(3):e2302287. doi: 10.1002/adhm.202302287. Epub 2023 Nov 15.
Wound healing is a well-orchestrated progress associated with angiogenesis, epithelialization, inflammatory status, and infection control, whereas these processes are seriously disturbed in diabetic wounds. In this study, a biohybrid dressing integrating the inherent ability of Bromeliad leaf (photosynthesis and self-draining) with the therapeutic effect of artificial materials (glucose-degrading and ROS-scavenging) is presented. The dressing consists of double-layered structures as follows: 1) Outer layer, a Bromeliad leaf substrate full of alginate hydrogel-immobilized glucose oxidase (GOx@Alg@Bromeliad substrate, abbreviated as BGA), can generate oxygen to guarantee the GOx-catalyzed glucose oxidation by photosynthesis, reducing local hyperglycemia to stabilize hypoxia inducible factor-1 alpha (HIF-1α) for angiogenesis and producing hydrogen peroxide for killing bacteria on the surface of wound tissue. The sophisticated structure of the leaf drains excessive exudate away via transpiration-mimicking, preventing skin maceration and impeding bacterial growth. 2) Inner layer, microneedles containing catalase (CAT-HA MNs, abbreviated as CHM), reduces excessive oxidative stress in the tissue to promote the proliferation of fibroblasts and inhibits proinflammatory polarization of macrophages, improving re-epithelialization of diabetic wounds. Together, the biohybrid dressing (BGA-CHM, abbreviated as BCHM) can enhance angiogenesis, strengthen re-epithelialization, alleviate chronic inflammation, and suppress bacterial infection, providing a promising strategy for diabetic wound therapy.
伤口愈合是一个协调良好的过程，涉及血管生成、上皮化、炎症状态和感染控制，而这些过程在糖尿病伤口中严重受到干扰。在这项研究中，提出了一种生物杂化敷料，它将凤梨科植物叶子的固有能力（光合作用和自排水）与人工材料的治疗效果（葡萄糖降解和 ROS 清除）结合在一起。该敷料由双层结构组成：1）外层，充满了固定化葡萄糖氧化酶的藻酸盐水凝胶的凤梨科植物叶子基质（GOx@Alg@Bromeliad substrate，简称 BGA），可以产生氧气，保证 GOx 催化的葡萄糖氧化作用，通过光合作用降低局部高血糖，稳定缺氧诱导因子-1α（HIF-1α）以促进血管生成，并产生过氧化氢杀死伤口组织表面的细菌。叶子的复杂结构通过模仿蒸腾作用将过多的渗出液排出，防止皮肤浸渍和细菌生长。2）内层，含有过氧化氢酶的微针（CAT-HA MNs，简称 CHM），减少组织中过多的氧化应激，促进成纤维细胞的增殖，并抑制巨噬细胞的促炎极化，改善糖尿病伤口的再上皮化。总之，生物杂化敷料（BGA-CHM，简称 BCHM）可以增强血管生成，加强再上皮化，减轻慢性炎症，抑制细菌感染，为糖尿病伤口治疗提供了一种有前途的策略。
