Graduate Program in Biotechnology, University of Taquari Valley - Univates, Lajeado, Brazil.
Graduate Program in Veterinary Medicine, Federal University of Santa Maria - UFSM, Santa Maria, Brazil.
World J Microbiol Biotechnol. 2023 Nov 5;40(1):5. doi: 10.1007/s11274-023-03820-z.
Feeding animals with lactobacilli strains is a biotechnological strategy to improve production, food quality, and animal health. Thus, this study aimed to select new lactic acid bacteria (LAB) able to improve laying hens health and egg production. Forty Bovans White layers (two days old) were randomly divided into four experimental groups that receive an oral gavage with saline solution (control group) or with one of the three lactobacilli selected (KEG3, TBB10, and KMG127) by their antagonistic activity against the foodborne pathogen Bacillus cereus GGD_EGG01. 16 S rRNA sequencing identified KEG3 as Lentilactobacillus sp., and TBB10 and KMG127 as Lactiplantibacillus sp. The data showed that feeding birds with LAB increased weight uniformity and improved the internal quality of the eggs (high yolk index and Haugh unit) compared with the control group (p < 0.05). Beta-diversity analysis showed that LAB supplementation modifies the cecal microbiota of laying hens. The prokaryotic families Bacteroidaceae, Ruminococcaceae, Rikenellaceae, and Lactobacillaceae were most important to the total dissimilarity of the cecal microbial community (calculated by SIMPER test). At end of in vivo experiments, it was possible to conclude that the feed of laying hens with Lentilactobacillus sp. TBB10 and Lentilactobacillus sp. KEG3 can be an important biotechnological tool for improving food quality and animal health.
用乳酸菌菌株喂养动物是一种生物技术策略，可提高生产、食品质量和动物健康。因此，本研究旨在选择新的乳酸菌（LAB），以改善蛋鸡的健康和产蛋量。40 只博万斯白层（两天大）被随机分为四个实验组，分别用生理盐水（对照组）或三种拮抗菌株（KEG3、TBB10 和 KMG127）进行口服灌胃，这些拮抗菌株对食源性病原体蜡样芽胞杆菌 GGD_EGG01 具有拮抗活性。16S rRNA 测序鉴定 KEG3 为 Lentilactobacillus sp.，TBB10 和 KMG127 为 Lactiplantibacillus sp.。数据显示，与对照组相比，用 LAB 喂养鸟类可提高体重均匀度并改善鸡蛋的内部质量（蛋黄指数和哈夫单位高）（p<0.05）。β多样性分析表明，LAB 补充剂改变了产蛋鸡的盲肠微生物群。拟杆菌科、瘤胃球菌科、真杆菌科和乳杆菌科是盲肠微生物群落总差异的最重要因素（通过 SIMPER 测试计算）。在体内实验结束时，可以得出结论，用 Lentilactobacillus sp. TBB10 和 Lentilactobacillus sp. KEG3 喂养产蛋鸡的饲料可能是改善食品质量和动物健康的重要生物技术工具。
