Animal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Sichuan Agricultural University, Chengdu 611130, China.
DSM Nutritional Products, Animal Nutrition and Health, Columbia, MD, USA.
Poult Sci. 2021 Jan;100(1):196-205. doi: 10.1016/j.psj.2020.09.065. Epub 2020 Oct 7.
This study was conducted to determine the effects of supplemental dietary benzoic acid on production performance, egg quality, intestinal morphology, and intestinal microbiota of laying hens. A total of seven hundred twenty 45-wk-old Lohman pink-shell laying hens were randomly allocated to 3 dietary treatments: control (CON), diet supplemented with 1,000 mg/kg benzoic acid (BA1), and 2,000 mg/kg benzoic acid (BA2). Each treatment included 10 replicates of 24 hens; laying hens were monitored for 16 wk. Overall, the results indicate that benzoic acid supplementation had no effect on laying rate, feed intake, feed conversion ratio, and breaking rate; however, a decrease in egg weight (P < 0.01) was observed in the BA2 group. Albumen height and Haugh unit (HU) were also linearly increased in the BA1 and BA2 groups (linear effect, P < 0.05). An increase in duodenum villus height (V) (quadratic effect, P = 0.041) and crypt depth (C) (linear effect, P = 0.012) was observed in the BA2 group, whereas an increased jejunum C and decreased V/C (quadratic effect, P < 0.05) in the BA1 group. Moreover, an increase in ileum V and C (quadratic effect, P < 0.05) was observed in the BA1 group. Microbial richness and diversity were reduced in the BA2 group (P < 0.01). An increase in the abundance of Clostridia (class), Clostridiales (order), Ruminococcaceae (family), and Lachnospiraceae (family) was noted in the BA1 group, whereas an enrichment of Bacteroides caecicola (species) was observed in the BA2 group. The HU positively correlated with genus Sphaerochaeta and Enorma (r = 0.56, 0.56; P < 0.05) but negatively correlated with Romboutsia, Subdoligranulum, Helicobacter, and Mucispirillum (r = -0.58, -0.49, -0.48; -0.70; P < 0.05). In conclusion, dietary supplementation with benzoic acid had no effect on production performance, but it significantly improved egg quality. In addition, 1,000 mg/kg benzoic acid positively modulated intestinal health by improving intestinal morphology and enriching microbial composition.
本研究旨在确定补充膳食苯甲酸对产蛋性能、蛋品质、肠道形态和肠道微生物的影响。将 720 只 45 周龄的罗曼粉壳蛋鸡随机分配到 3 种日粮处理组：对照组（CON）、日粮添加 1000mg/kg 苯甲酸（BA1）和 2000mg/kg 苯甲酸（BA2）。每个处理组包括 10 个 24 只母鸡的重复；监测产蛋鸡 16 周。总体而言，结果表明，苯甲酸的添加对产蛋率、采食量、饲料转化率和破蛋率没有影响；然而，BA2 组的蛋重下降（P < 0.01）。BA1 和 BA2 组的蛋清高度和哈格单位（HU）也呈线性增加（线性效应，P < 0.05）。BA2 组十二指肠绒毛高度（V）增加（二次效应，P = 0.041）和隐窝深度（C）增加（线性效应，P = 0.012），而 BA1 组空肠 C 增加和 V/C 减少（二次效应，P < 0.05）。此外，BA1 组回肠 V 和 C 增加（二次效应，P < 0.05）。BA2 组微生物丰富度和多样性降低（P < 0.01）。BA1 组梭菌（属）、梭状芽孢杆菌（目）、真杆菌科（科）和毛螺菌科（科）的丰度增加，而 BA2 组拟杆菌 caecicola（种）的丰度增加。HU 与 Sphaerochaeta 和 Enorma 呈正相关（r = 0.56，0.56；P < 0.05），与 Romboutsia、Subdoligranulum、Helicobacter 和 Mucispirillum 呈负相关（r = -0.58，-0.49，-0.48；-0.70；P < 0.05）。综上所述，日粮中添加苯甲酸对产蛋性能没有影响，但显著提高了蛋品质。此外，1000mg/kg 苯甲酸通过改善肠道形态和丰富微生物组成来改善肠道健康。
