McConville M L, Charles H P
J Gen Microbiol. 1979 Mar;111(1):193-200. doi: 10.1099/00221287-111-1-193.
Mutants of Escherichia coli K12 which accumulated the haem precursor porphobilinogen are described. The mutants grew very slowly on carbon and energy sources which K12 uses only oxidatively, and they had low catalase activities, suggesting that they were deficient in haem. Extracts had one-tenth of the parental activity of the enzyme porphobilinogen deaminase. In transduction, the mutation mapped close to genes ilvD and metE at minute 84. The gene was tentatively identified as hemC, coding for porphobilinogen deaminase. The gene symbol hemC replaces the earlier and temporary symbol popE.
本文描述了积累血红素前体胆色素原的大肠杆菌K12突变体。这些突变体在K12仅通过氧化方式利用的碳源和能源上生长非常缓慢，并且它们的过氧化氢酶活性较低，这表明它们缺乏血红素。提取物中胆色素原脱氨酶的活性仅为亲本的十分之一。在转导过程中，该突变位于84分钟处，靠近ilvD和metE基因。该基因被初步鉴定为hemC，编码胆色素原脱氨酶。基因符号hemC取代了早期的临时符号popE。
