Săsărman A, Chartrand P, Lavoie M, Tardif D, Proschek R, Lapointe C
J Gen Microbiol. 1979 Aug;113(2):297-303. doi: 10.1099/00221287-113-2-297.
A new type of haem-deficient mutant was isolated in Escherichia coli K12 by neomycin selection. The mutant, designated SASX38, accumulated uroporphyrin, coproporphyrin and protoporphyrin. Since it possessed normal ferrochelatase activity, it was assumed to be deficient in protoporphyrinogen oxidase activity. The gene affected in the mutant was designated hemG. Mapping of the hemG gene by phage P1-mediated transduction showed that it was located very close to the chlB gene (frequency of cotransduction 78.7%), between the metE and rha markers. This location is distinct from the other known hem loci in E. coli K12.
通过新霉素筛选在大肠杆菌K12中分离出一种新型的血红素缺陷型突变体。该突变体命名为SASX38，积累了尿卟啉、粪卟啉和原卟啉。由于它具有正常的亚铁螯合酶活性，推测其原卟啉原氧化酶活性不足。该突变体中受影响的基因命名为hemG。通过噬菌体P1介导的转导对hemG基因进行定位，结果表明它非常靠近chlB基因（共转导频率为78.7%），位于metE和rha标记之间。此位置与大肠杆菌K12中其他已知的血红素基因座不同。
